Accelerate to discover

Back to filter

Related topics

Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs

Fluxion Biosciences

Oct 12, 2018

Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...

Target large genomic regions with 10x genomics + SageHLS

Sage Science

Oct 9, 2018

With customized Cas9 nucleases, scientists can now isolate specific large genomic DNA fragments with the SageHLS...

Coy anaerobic chambers #1 choice in microbiology

COY laboratory products

Oct 5, 2018

Used for over 40 years in anaerobic microbiology research, COY chambers are also critical to many other research areas...

Understanding subtle cardiac changes

VisualSonics FujiFilm

Oct 3, 2018

Are you working with high ultrasound imaging and are you interesting in VevoStrain application and specific changes in...

Oct 16, 2018

EasyTEL+ unique digital platform for single or group-housed subjects e

emka TECHNOLOGIES

Sep 24, 2018

EMKA announced major enhancements of its easyTEL+ digital implanted telemetry. No more antennas inside cages,...

A carboxymethyl dextran-based polymeric conjugate as the antigen carrier for cancer immunotherapy

Spectral Instruments Imaging

Sep 18, 2018

A polymeric conjugate, composed of a carboxymethyl dextran (CMD) as the backbone and ovalbumin (OVA) as a model foreign...

Adenovirus type B detection using the xxpress qPCR thermal cycler

BJS Biotechnologies

Sep 17, 2018

The ultrafast xxpress qPCR thermal cycler through performing standard dilution series and multiplex qPCR experiments is...

Oct 16, 2018

Contrast-enhanced ultrasound imaging

VisualSonics FujiFilm

Sep 5, 2018

Are you interested in photoacoustic imaging and would like to enhance your contrast imaging acquisitions? Just use...

Show all topics (10)

Circulating Tumor Cell cultures (CTC) under pressure using Avatar bioreactor

Oct 17, 2017

The Avatar system from Xcell Biosciences was originally designed to maintain and propagate human stem cells under culturing conditions that replicate the stem cell niche. The system was modified to cultivate a variety of primary human tumor cells, including CTCs, by replicating conditions found within the tumor microenvironment. The Avatar system incorporates a unique bioreactor that regulates settings for oxygen and atmospheric pressure levels, in addition to the usual temperature and carbon dioxide controls. Complementing the novel bioreactor are serum-free, chemically defined culture media composed of recombinant human growth factors, and a CTC-capture substrate composedof a collagen-based hydrogel.

The workflow was developed in response to mounting evidence that hypoxia and pressure are influential traits in the cellular microenvironment that contribute to the maintenance and propagation of tumorcells. When complemented with an optimal cocktail of growth factors and a collagen-rich substrate found within tumor tissues, tumor cells can be maintained and propagated ex vivo, at rates similar to those observed in the body. Other studies investigatingcell morphology as well as gene and protein expression profiles have shown that cells cultured in the Avatar system more faithfully reflect in vivo biology than cells grown in traditional CO2 incubators supplemented with animal serum-based culture media.

Read more

Scientific paper
Product news
Video

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey