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Design and analysis of single-cell sequencing experiments

Apr 21, 2016

Recent advances in single-cell sequencing hold great potential for exploring biological systems
with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations
and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can
elucidate the cell type composition of a sample. However, single-cell sequencing comes with major
technical challenges and yields complex data output. In this Primer, we provide an overview of
available methods and discuss experimental design and single-cell data analysis. We hope that
these guidelines will enable a growing number of researchers to leverage the power of single-cell
sequencing.

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Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

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