Accelerate to discover

Back to filter

Related topics

STAT Protein Phosphorylation in CD4+ T Cells using the CellStream flow cytometer

Luminex

Jan 21, 2021

Cytokine-induced protein phosphorylation is a key cell signaling event within the immune system that modulates diverse...

Introducing Cytek cFluor, 14 Colors Immunoprofiling Kit

Cytek Biosciences

Jan 19, 2021

Cytek cFluor Immunoprofiling Kit allows for the identification of helper T cells, cytotoxic T cells, B cells, NK cells...

A Glimpse on physiological monitoring for the in vivo SKYSCAN Systems

Bruker Biospin

Jan 14, 2021

This past year has proven once again that in vivo imaging is an important aspect in the microCT world. For longitudinal...

Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting

Cytek Biosciences

Jan 11, 2021

In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...

Jan 25, 2021

CQ1 webinar : optimizing high-content imaging of 3D models for drug discovery

Yokogawa

Jan 7, 2021

In vitro spheroid models are fast becoming the de facto standard for drug discovery applications, largely due to their...

IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate

Sartorius

Jan 4, 2021

Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte platform for the in-vitro...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 28, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Jan 25, 2021

EV imaging series: Using ONI super-resolution to characterize single-EVs

ONI

Dec 23, 2020

To date, researchers commonly use flow-based characterization methods, fluorescence imaging techniques and electron...

Show all topics (10)

Digital measurement for HER2 amplifcation in HER2 equivocal breast cancer diagnosis

Sep 20, 2017

Currently, there are no recommended alternative assays for HER2 cases deemed equivocal by immunohistochemistry and fuorescent in situ hybridization. Digital PCR (ddPCR), a highly accurate method to determine DNA copy number, could be a robust alternative for clinical HER2 diagnostics. HER2 and CEP17 copy numbers were quantifed using two ddPCR platforms (QX200 and RainDrop) in 102 samples of invasive breast cancers. Compared to routine assays, ddPCR gave a sensitivity
and specifcity of 82.8% and 97.3% respectively, with a kappa value of 0.833 (p<0.001). Moreover, the method proved to be robust as the results from two platforms was highly correlated R2=0.91; Concordance rate=97%; κ=0.923, P<0.001). Its performance was further tested on 114 HER2 equivocal cases in an independent validation cohort. 75% (21/28) of cases with HER2 amplifcation and 95% (82/86) of HER2 non-amplifed case were classifed as positive and negative by ddPCR respectively (κ=0.709, P<0.001). Notably, in the HER2 amplifed cases, a lower percentage of HER2 positive cells could be related to the discordant results. Altogether, ddPCR is a robust alternative for clinical HER2 diagnostics. However, intratumoral heterogeneity of HER2 status still pose a challenge for HER2 analysis by ddPCR.

Read more

Scientific paper
Application

Related technologies: Digital PCR

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

RainDance Technologies

RainDance company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases.

Related products

Capable of generating more than a billion reactions in a single day, the RainDrop system surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing.

show detail

A fast, accurate and low-cost NGS target enrichment system that features award-winning instrumentation and pre-validated gene panels, as well as flexible open source options.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey