Accelerate to Discover

Back to filter

Related topics

Method to quantify local force distribution within biomolecular systems

LUMICKS

May 24, 2018

The journal Nano Letters has recently published the article “Quantifying Local Molecular Tension Using Intercalated DNA...

Registering FDG/PET mouse and AMYViD/PET-CT rat studies to brain VOI atlases

Bruker Biospin

May 22, 2018

Preclinical NeuroPET is employed widely in studies of stroke, ischemia, addiction and eurodegenerative diseases. In...

Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation

Etaluma

May 21, 2018

Adverse tissue responses to metal wear and corrosion products from CoCr alloy implants remain a great challenge to...

New protocol: Analyze small extracellular RNAs with PippinHT

Sage Science

May 18, 2018

We’re pleased to see that PippinHT is featured heavily in the protocol for DNA sizing steps following PCR...

May 28, 2018

Genoox, Bionano collaborate to enhance the detection of pathogenic SVs

BioNano Genomics

May 14, 2018

Bionano is now working with the global health company Genoox to develop a genome informatics workflow to integrate and...

Outstanding return on investment with the xxpress qPCR thermal cycler

BJS Biotechnologies

May 10, 2018

Find out just how much money you could save by switching to the xxpress qPCR thermal cycler,visit the cost savings...

AVATAR Improves transfection efficiency with high viability

Xcell Biosciences

May 9, 2018

AVATAR lets you create any cell type even novel ones, with efficient transfection that improves reprogramming rates...

May 28, 2018

Creating a bold new future in ultrasound and photoacoustics

VisualSonics FujiFilm

May 4, 2018

Fujifilm VisualSonics has been chosen as one of the top 10 visualization solution providers in the field of developing...

Show all topics (10)

Digital measurement for HER2 amplifcation in HER2 equivocal breast cancer diagnosis

Sep 20, 2017

Currently, there are no recommended alternative assays for HER2 cases deemed equivocal by immunohistochemistry and fuorescent in situ hybridization. Digital PCR (ddPCR), a highly accurate method to determine DNA copy number, could be a robust alternative for clinical HER2 diagnostics. HER2 and CEP17 copy numbers were quantifed using two ddPCR platforms (QX200 and RainDrop) in 102 samples of invasive breast cancers. Compared to routine assays, ddPCR gave a sensitivity
and specifcity of 82.8% and 97.3% respectively, with a kappa value of 0.833 (p<0.001). Moreover, the method proved to be robust as the results from two platforms was highly correlated R2=0.91; Concordance rate=97%; κ=0.923, P<0.001). Its performance was further tested on 114 HER2 equivocal cases in an independent validation cohort. 75% (21/28) of cases with HER2 amplifcation and 95% (82/86) of HER2 non-amplifed case were classifed as positive and negative by ddPCR respectively (κ=0.709, P<0.001). Notably, in the HER2 amplifed cases, a lower percentage of HER2 positive cells could be related to the discordant results. Altogether, ddPCR is a robust alternative for clinical HER2 diagnostics. However, intratumoral heterogeneity of HER2 status still pose a challenge for HER2 analysis by ddPCR.

Read more

Scientific paper
Application

Related technologies: Digital PCR

Get more info

Regina Fillerová

Field Application Specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

RainDance Technologies

RainDance company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases.

Related products

Capable of generating more than a billion reactions in a single day, the RainDrop system surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing.

show detail

A fast, accurate and low-cost NGS target enrichment system that features award-winning instrumentation and pre-validated gene panels, as well as flexible open source options.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey