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Digital measurement for HER2 amplifcation in HER2 equivocal breast cancer diagnosis

Sep 20, 2017

Currently, there are no recommended alternative assays for HER2 cases deemed equivocal by immunohistochemistry and fuorescent in situ hybridization. Digital PCR (ddPCR), a highly accurate method to determine DNA copy number, could be a robust alternative for clinical HER2 diagnostics. HER2 and CEP17 copy numbers were quantifed using two ddPCR platforms (QX200 and RainDrop) in 102 samples of invasive breast cancers. Compared to routine assays, ddPCR gave a sensitivity
and specifcity of 82.8% and 97.3% respectively, with a kappa value of 0.833 (p<0.001). Moreover, the method proved to be robust as the results from two platforms was highly correlated R2=0.91; Concordance rate=97%; κ=0.923, P<0.001). Its performance was further tested on 114 HER2 equivocal cases in an independent validation cohort. 75% (21/28) of cases with HER2 amplifcation and 95% (82/86) of HER2 non-amplifed case were classifed as positive and negative by ddPCR respectively (κ=0.709, P<0.001). Notably, in the HER2 amplifed cases, a lower percentage of HER2 positive cells could be related to the discordant results. Altogether, ddPCR is a robust alternative for clinical HER2 diagnostics. However, intratumoral heterogeneity of HER2 status still pose a challenge for HER2 analysis by ddPCR.

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RainDance Technologies

RainDance company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases.

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Capable of generating more than a billion reactions in a single day, the RainDrop system surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing.

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A fast, accurate and low-cost NGS target enrichment system that features award-winning instrumentation and pre-validated gene panels, as well as flexible open source options.

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