Accelerate to Discover

Back to filter

Related topics

New technologies to uncover the molecular basis of disorders of sex development

BioNano Genomics

Jul 13, 2018

Newer approaches, such as whole-genome sequencing and whole-genome mapping have the potential to identify novel...

δT cells and CD8+ T cells targeting of breast cancer cells

Etaluma

Jul 11, 2018

Cancer stem cells (CSCs) are a large cause of metastasis in breast cancer. They are also very resistent to any...

Incucyte system used for generating single-cell-derived cancer-associated fibroblast clones

Sartorius

Jul 9, 2018

Single-cell-derived cancer-associated fibroblast (CAFs) were produced using Incucyte. These cells were used to help...

Real-time verification of clonality using IncuCyte system

Sartorius

Jun 28, 2018

Use whole-well, label-free imaging to automatically scan for clones and verify monoclonality. Integrated image...

Jul 17, 2018

First DLS paper: Sorghum genome assembled with nanopore sequencing and DLS

BJS Biotechnologies

Jun 26, 2018

The new paper describes a chromosome-scale de novo assembly of the repeat-rich Sorghum bicolor Tx430 genome built using...

Real-time study of platelet formation under sheer-flow with Lumascope

Etaluma

Jun 18, 2018

Platelet formation was observed with Lumascope microscope in a uniform-sheer-flow biorector. Videos of the formation...

Early fetal sex determination using micro-volume of maternal plasma

BJS Biotechnologies

Jun 16, 2018

 A qPCR-based assay, SneakPeek Early Gender Test (Gateway Genomics) has been developed to determine fetal sex as early...

Jul 17, 2018

3R Compliant drug development studies

Bruker Biospin

Jun 8, 2018

In preclinical imaging, magnetic resonance imaging (MRI) can be an attractive alternative to researchers who have...

Show all topics (10)

Droplet-based digital PCR: Application in cancer research

Apr 11, 2017

The efficient characterization of genetic and epigenetic alterations in oncology, virology, or prenatal diagnostics requires highly sensitive and specific high-throughput approaches. Nevertheless, with the use of conventional methods, sensitivity and specificity were largely limited. By partitioning individual target molecules within distinct compartments, digital PCR (dPCR) could overcome these limitations and detect very rare sequences with unprecedented precision and sensitivity. In dPCR, the sample is diluted such that each individual partition will contain no more than one target sequence. Following the assay reaction, the dPCR process provides an absolute value and analyzable quantitative data. The recent coupling of dPCR with microfluidic systems in commercial platforms should lead to an essential tool for the management of patients with cancer, especially adapted to the analysis of precious samples. Applications in cancer research range from the analysis of tumor heterogeneity to that of a range of body fluids. Droplet-based dPCR is indeed particularly appropriate for the emerging field of liquid biopsy analysis. In this review, following an overview of the development in dPCR technology and different strategies based on the use of microcompartments, we will focus particularly on the applications and latest development of microfluidic droplet-based dPCR in oncology.

Read more

Scientific paper
Application

Related technologies: Digital PCR

Get more info

Regina Fillerová

Field Application Specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

RainDance Technologies

RainDance company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases.

Related products

Capable of generating more than a billion reactions in a single day, the RainDrop system surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing.

show detail

A fast, accurate and low-cost NGS target enrichment system that features award-winning instrumentation and pre-validated gene panels, as well as flexible open source options.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey