Liquid biopsy assays have shown great promise as a precision medicine approach to therapy selection and patient monitoring, but significant sensitivity and specificity improvements are still needed. To address this we introduce ERASE-Seq (Elimination of recurrent artifacts and stochastic errors), a method for accurate and sensitive detection of ultra-low frequency DNA variants.
ERASE-Seq differs from previous methods by creating a variant event matrix that includes multiple sample runs and control data. A robust statistical framework is applied at each position in the sequence, providing a 10 to 100-fold reduction in false positive rates compared to published molecular barcoding methods.
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