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Evaluation of quantum dot toxicity on three-dimensional spheroid using Lumascope720

Apr 12, 2018

Morphological investigations and time-resolved microscopy analysis in addition to cell metabolic activity studies revealed that 3D spheroid cultures are more resistant to Qdot-induced cytotoxicity in comparison to conventional 2D cultures. The obtained results suggest the presence of two distinct cell populations in 2D cultures with different sensitivity to Qdots, however that effect wasn't observed in 3D spheroids. Our investigations were aimed to improve the prediction of nanotoxicity of Qdot on tissue-level and provide the essential screening steps prior to any in vivo application. Moreover, penetration ability of highly fluorescent Qdots to densely-packed spheroids will fortify the biological application of developed Qdots in tissue-like structures.

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Related technologies: Live cell imaging

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Etaluma

With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.

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The original single color 488nm excitation fluorescence inverted microscopy

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