S2 Genomics has developed the patented Singulator™ 100 and 200 Systems to automate the dissociation of solid tissues into single cell or nuclei suspensions in single-use cartridges
using coupled enzymatic or chemical dissociation and mechanical disruption.
Here we demonstrate the utility of the Singulator platform for the isolation of nuclei from plant
embryos, and the impact of the improved nuclei quality over manually prepared nuclei on single nuclei
sequencing, enabling the identification of a candidate marker gene associated with the B Chromosome in
a specific cell type that may be related to the regulation of the B Chromosome elimination during
embryonic development.
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