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Fluorescent reagents for in vivo optical imaging

May 26, 2017

Applications for in vivo fluorescent small animal imaging are varied and include cell tracking, tracer/probe development, and detection of molecular biomarkers. These methods have been applied in models of cancer, infection, inflammation, and stem cell biology. The catalogue of fluorescent reagents has expanded significantly in the past decade.
There have been two primary developments in the evolution of in vivo fluorescent reagents during this time (1) red-shifted fluorescent proteins (e.g. tdTomato) and NIR dyes (e.g. Cy7), which are more commonly used for in vivo imaging applications and (2) the range of validated targeted probes, which has expanded significantly.

Bruker Optical/X-ray imaging systems are equipped standard with full multispectral (Yudina et al., 2016) fluorescence VISNIR imaging capability, compatible with most custom and commercial reporters.
Also, Bruker Optical/X-ray imaging systems have been employed with a range of fluorescent reporters including but not limited to YFP, eGFP, mCherry, and many others.

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