IntelliCyt Corporation's high throughput flow technology is the first to enable screening suspension cells, beads, microbes and mixtures.
Accelerate to discover
Related topics
InAlyzer to evaluate G6PD activity in relation with frailty
Mar 28, 2023
InAlyzer is body densitometry instrument for lab animals, equipped with 2 X-Ray sources and able to provide valuable...
Cardiomyocyte isolation using Cellenion’s cellenONE instrument
Mar 24, 2023
The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...
ACCELA educational webinar: Unlocking Spatial Biology with RareCyte Orion
Mar 16, 2023
Experience Spatial Biology with Orion Multiplex Imaging. Orion is a benchtop, high...
Recorded webinar: Anesthesia Considerations in Small Animal Imaging
Mar 13, 2023
Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...
Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay
Mar 13, 2023
This kit has been designed to enumerate all major leukocyte subsets and it mirrors and expands on those identified in a...
Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II
Mar 7, 2023
CyteFinder II Instruments are high speed, whole slide imaging systems with options for liquid biopsy analysis and...
NanoCellect Webinar: Optimizing the Cell Line Development Process with Microfluidic Cell Sorting
Mar 3, 2023
Single-cell selection and cloning are required for bioengineering workflows such as antibody production, cell therapy,...
New Kit for Bacterial analysis expands capability of Guava Muse flow cytometer
Feb 20, 2023
With the Guava Muse cell analyzer, you can now achieve highly quantitative results at a fraction of the price, effort,...
Sep 14, 2016
Traditional assays for monitoring cell-mediated killing are only capable of homogenous live/dead
readouts for an entire sample. IntelliCyt’s iQue Screener is a high throughput suspension screening
platform based on flow cytometry. The system can identify multiple cell types in solution and report on
multiple cell killing readouts, including cell viability and apoptosis. Here we demonstrate high
throughput flow assays for cell-mediated killing in examples using NK cells and chimeric antigen
receptor (CAR) T-cells. For the NK cell assay, NK92 cells were utilized as effector cells and Jurkat
cells were utilized as target cells. By labeling either target or effector cells with IntelliCyt’s MultiCyt FL4
Cell Proliferation and Encoder dye, both cell populations can be simultaneously monitored. Viability
was determined by cell membrane integrity and Caspase 3 activation for both Jurkat and NK92 cells,
and the specificity of the killing response was demonstrated using inhibitors of cell killing activity. In the
CAR T-cell assay, the efficacy of different CARs at targeting and killing a B-cell line (NALM-6) was
profiled with multiplex assays. In addition to cell encoding and live/dead analysis, cytokine responses
were evaluated using bead-based ELISA on the same analysis platform. These examples highlight the
robustness and flexibility of the iQue Screener for performing multiplexed screening assays for cell
mediated killing with greater contextual value.
Related technologies: HTP Screening
Get more info
Brand profile
IntelliCyt Corporation's high throughput flow technology is the first to enable screening suspension cells, beads, microbes and mixtures.
More info at:
www.intellicyt.com/