Accelerate to discover

Back to filter

Related topics

Cell plasticity associated to circulating tumor cells from prostate cancer patients

Fluxion Biosciences

Mar 13, 2019

Treatment resistance is associated with tumor cells molecular plasticity. In metastatic castration resistant prostate...

Izon qEV - The fastest & most effective Exosome isolation

IZON

Mar 6, 2019

Extracellular  vesicles  (EVs)  are  prevalent  in biological  fluids  and  are  of  great  interest  to researchers ...

Enhanced uptake of luminescent quantum dots by live cells

Etaluma

Mar 1, 2019

The steady progress made over the past threedecades in growing a variety of inorganic nanomaterials, with discreet...

Fat-water separation Imaging in Preclinical MRI

Bruker Biospin

Mar 1, 2019

Non-invasive in vivo phenotyping of adipose tissue deposits in small animal models is of great importance in...

Mar 19, 2019

Precision CellRad

Precision X-Ray

Feb 15, 2019

The CellRad system allows you to irradiate cells in your lab without compromising sterility or security. A smaller,...

New technique for dynamic monitoring of liquid tumor Cytotoxicity

Acea Biosciences

Feb 12, 2019

To develop and validate immuno-oncology therapies, scientists must understand the full dynamics of cytotoxic effectors...

Small animal 13C-hyperpolarized MRI

Bruker Biospin

Feb 8, 2019

13C-Hyperpolarized molecules provide a unique signature for molecular processes relevant to a range of disease...

Mar 19, 2019

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer xenografts

Spectral Instruments Imaging

Jan 30, 2019

Pancreatic ductal adenocarcinoma has been increasing by 0.5% per year in the United States. This study describes the...

Show all topics (10)

High throughput flow screening assays to profile cell-mediated killing

Sep 14, 2016

Traditional assays for monitoring cell-mediated killing are only capable of homogenous live/dead
readouts for an entire sample. IntelliCyt’s iQue Screener is a high throughput suspension screening
platform based on flow cytometry. The system can identify multiple cell types in solution and report on
multiple cell killing readouts, including cell viability and apoptosis. Here we demonstrate high
throughput flow assays for cell-mediated killing in examples using NK cells and chimeric antigen
receptor (CAR) T-cells. For the NK cell assay, NK92 cells were utilized as effector cells and Jurkat
cells were utilized as target cells. By labeling either target or effector cells with IntelliCyt’s MultiCyt FL4
Cell Proliferation and Encoder dye, both cell populations can be simultaneously monitored. Viability
was determined by cell membrane integrity and Caspase 3 activation for both Jurkat and NK92 cells,
and the specificity of the killing response was demonstrated using inhibitors of cell killing activity. In the
CAR T-cell assay, the efficacy of different CARs at targeting and killing a B-cell line (NALM-6) was
profiled with multiplex assays. In addition to cell encoding and live/dead analysis, cytokine responses
were evaluated using bead-based ELISA on the same analysis platform. These examples highlight the
robustness and flexibility of the iQue Screener for performing multiplexed screening assays for cell
mediated killing with greater contextual value.

Read more

Scientific paper
Application
Product news

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

IntelliCyt

IntelliCyt Corporation's high throughput flow technology is the first to enable screening suspension cells, beads, microbes and mixtures.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey