Accelerate to discover

Back to filter

Related topics

Discover the Celloger live-cell analyzers : miniaturized and powerful tools for every Biologist

Curiosis

Apr 5, 2024

Celloger live-cell analyzers, image and analyze your cells in real-time, inside your incubator. With its exceptional...

The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging

Precision X-Ray

Apr 4, 2024

Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging...

A microthrombus-driven fixed-point cleaved nanosystem for preventing post-thrombolysis recurrence

IVIM Technology

Apr 3, 2024

A thrombin-responsive and fixed-point cleaved Fu@pep-CLipo was developed for highly efficient and precise thrombolysis...

Webinar: Multimodal Assessment of Hypoxia in Tumors: From the Lab to the Clinic

Bruker Biospin

Apr 2, 2024

25 April 2024, 4PM CET
This webinar will be of interest to multiple profiles in the community of biomedical
...

Apr 20, 2024

Multiplexed tissue imaging using the Orion platform to reveal the Spatial Biology of Cancer

RareCyte

Mar 27, 2024

In this webinar Prof. Sandro Santagata, will reveal how Orion high-plex imaging and the use of this data, is valuable...

Cytek SpectroFlo Software Version 3.3 has been released!

Cytek Biosciences

Mar 25, 2024

SpectroFlo Software Version 3.3 has been released, enabling new assays with SpectroFlo Software version 3.3, including...

A 19-color single-tube Full Spectrum Flow Cytometry for the detection of Acute Myeloid Leukemia

Cytek Biosciences

Mar 13, 2024

This recent publication in Cytometry Part A describes the development and comprehensive workflow of a single-tube,...

Apr 20, 2024

18F-labeled somatostatin analogs for somatostatin receptors (SSTRs) targeted PET imaging of NETs

MOLECUBES

Mar 11, 2024

A novel 18F-radiolabeled somatostatin analogue, [Al18F]NODA-MPAA-HTA, was synthesized and evaluated for positron...

Show all topics (10)

High throughput flow screening assays to profile cell-mediated killing

Sep 14, 2016

Traditional assays for monitoring cell-mediated killing are only capable of homogenous live/dead
readouts for an entire sample. IntelliCyt’s iQue Screener is a high throughput suspension screening
platform based on flow cytometry. The system can identify multiple cell types in solution and report on
multiple cell killing readouts, including cell viability and apoptosis. Here we demonstrate high
throughput flow assays for cell-mediated killing in examples using NK cells and chimeric antigen
receptor (CAR) T-cells. For the NK cell assay, NK92 cells were utilized as effector cells and Jurkat
cells were utilized as target cells. By labeling either target or effector cells with IntelliCyt’s MultiCyt FL4
Cell Proliferation and Encoder dye, both cell populations can be simultaneously monitored. Viability
was determined by cell membrane integrity and Caspase 3 activation for both Jurkat and NK92 cells,
and the specificity of the killing response was demonstrated using inhibitors of cell killing activity. In the
CAR T-cell assay, the efficacy of different CARs at targeting and killing a B-cell line (NALM-6) was
profiled with multiplex assays. In addition to cell encoding and live/dead analysis, cytokine responses
were evaluated using bead-based ELISA on the same analysis platform. These examples highlight the
robustness and flexibility of the iQue Screener for performing multiplexed screening assays for cell
mediated killing with greater contextual value.

Read more

Scientific paper
Application
Product news

Related technologies: HTP Screening

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

IntelliCyt

IntelliCyt Corporation's high throughput flow technology is the first to enable screening suspension cells, beads, microbes and mixtures.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey