Accelerate to discover

Back to filter

Related topics

Standardize tumor sample preparation and reduce background with Curiox laminar wash system

Curiox

Aug 10, 2022

Laminar Wash technology offers a gentle alternative that can improve yield and data quality, particularly of rare cell...

Developing a 43 color panel on Cytek Aurora using Laminar Wash for sample prep

Curiox

Aug 2, 2022

Successfully executing a 43 color panel is a technically demanding task. One that is made more difficult when there are...

Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids

Cellenion

Jun 21, 2022

spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....

Outstanding post-sort sample recovery on the WOLF Cell Sorter

NanoCellect

Jun 14, 2022

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...

Aug 18, 2022

Incucyte AI module: Advanced Label-Free Classification Analysis

Sartorius & Essen BioScience

Jun 6, 2022

Monitor adherent cell morphology changes objectively to determine live/dead cell counts or classify cells based on...

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters

MOLECUBES

Jun 3, 2022

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...

An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing

Sage Science

Jun 1, 2022

Recent article from February 2022 shoes how size selection improved viral read mapping by over 90% for begomoviruses.

Aug 18, 2022

Intoducing cellenONE: The revolutionary Single Cell isolator

Cellenion

May 25, 2022

A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...

Show all topics (10)

Inhibition of T cell receptor signaling mediated Calcium flux in T cells

Apr 23, 2018

Increased intracellular Ca2+ content is an essential and irreversible step towards the activation of T lymphocytes and indicated in regulating their cell survival, proliferation and differentiation. Upon antigen recognition, activation of the TCR initiates numerous signaling cascades resulting in cellular activation and proliferation. During TCR signaling, IL-2-inducible kinase (ITK) activates phospholipase C (PLC) and the subsequent release of intracellular Ca2+ stores from the endoplasmic reticulum (ER) resulting in a rapid elevation of intracellular Ca2+ levels up to 500nM. This process subsequently triggers the opening of ion channels in the plasma membrane allowing an influx of extracellular Ca2+, which in turn activates key molecules such as Calcineurin. Inhibition of calcium flux has been shown to affect T cell function, therefore, measuring the Ca2+ content in T cells presents important information about the status and kinetics of T cell activation. The capability of the NovoCyte flow cytometer to quantify dynamic changes in intracellular Ca2+ was demonstrated by measuring calcium flux in Jurkat T cells. This data demonstrates that dynamic alterations in intracellular Ca2+ concentration can be measured on the NovoCyte flow cytometer.

Read more!

Application
Product news

Related technologies: Conventional flow cytometry

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey