Accelerate to discover

Back to filter

Related topics

Why ventilator so important in the battle against COVID-19?

RWD

Sep 21, 2020

The COVID-19 has caused shortages of medical devices in many countries, especially ventilators. Many companies around...

Discover the newly re-designed TissueGnostics portfolio

TissueGnostics

Sep 18, 2020

TissueGnostics is a solution provider for Precision Medicine and provides fully integrated cutting-edge tissue...

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

Are you still using traditional methodologies for your Oncology cell metabolism studies?

Sartorius

Sep 10, 2020

In this infographic, learn about the challenges experienced by scientists in analysing cellular metabolism and how...

Sep 22, 2020

Using DNA repair and machine learning to improve cancer risk prediction

Luminex

Sep 7, 2020

Across the world, breast cancer continues to be the leading type of cancer in women, accounting for 25% of all cases....

Quantitative evaluation of pulmonary function with hyperpolarized xenon gas MR

Bruker Biospin

Aug 20, 2020

Hyperpolarized 129Xe MR has been proven to be a powerful tool to evaluate the pulmonary function and has been widely...

Get more out of your full spectrum panel with Cytek’s new reagent family

Cytek Biosciences

Aug 17, 2020

Cytek Biosciences has already redefined what is possible in flow cytometry. The new Cytek cFluor family of reagents...

Sep 22, 2020

Applications of Hyperpolarized 129Xe MRI for functional imaging of the lungs

Bruker Biospin

Aug 11, 2020

2015 marked the first ever translation of hyperpolarized gas MRI to diagnostic clinical practice. Research at the...

Show all topics (10)

Inhibition of T cell receptor signaling mediated Calcium flux in T cells

Apr 23, 2018

Increased intracellular Ca2+ content is an essential and irreversible step towards the activation of T lymphocytes and indicated in regulating their cell survival, proliferation and differentiation. Upon antigen recognition, activation of the TCR initiates numerous signaling cascades resulting in cellular activation and proliferation. During TCR signaling, IL-2-inducible kinase (ITK) activates phospholipase C (PLC) and the subsequent release of intracellular Ca2+ stores from the endoplasmic reticulum (ER) resulting in a rapid elevation of intracellular Ca2+ levels up to 500nM. This process subsequently triggers the opening of ion channels in the plasma membrane allowing an influx of extracellular Ca2+, which in turn activates key molecules such as Calcineurin. Inhibition of calcium flux has been shown to affect T cell function, therefore, measuring the Ca2+ content in T cells presents important information about the status and kinetics of T cell activation. The capability of the NovoCyte flow cytometer to quantify dynamic changes in intracellular Ca2+ was demonstrated by measuring calcium flux in Jurkat T cells. This data demonstrates that dynamic alterations in intracellular Ca2+ concentration can be measured on the NovoCyte flow cytometer.

Read more!

Application
Product news

Related technologies: Conventional flow cytometry

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey