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Label-free toxicology analysis

Aug 30, 2016

Cell counting, confluence and morphology are useful parameters for toxicity analysis. The HoloMonitor time-lapse cytometer determines cell proliferation, both by cell counting and by confluence, but also allows for quantitative assessments of cell morphology. Healthy cells are irregular and rather thin, dying cells are small, less irregular and thick, while dead cells are round and thin. These features are the basics for determination of drug effects on cell populations.

Using HoloMonitor, cell proliferation, cell morphology and cell viability can be measured at several time points. Individual cells and whole cell populations are analyzed at the same time. This makes it possible to conveniently evaluate several different toxicological parameters using very few cell samples. The data become more relevant when the same samples are studied, than when separate, replicate cultures
are used for different time points and parameters. This contributes to building statistically sound results.

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Phase Holographic Imaging

Holographic technology allows the long-term behavior of living cells to be observed and quantified without toxic stains.

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