Accelerate to discover

Back to filter

Related topics

Low-dose imaging in a new preclinical total-body PET/CT scanner

Bruker Biospin

Jun 12, 2019

Ionizing radiation constitutes a health risk to imaging scientists and study animals. Both PET and CT produce ionizing...

Fluxion Biosciences and National Cancer Institute cooperate to validate IsoFlux for sequencing

Fluxion Biosciences

Jun 12, 2019

Fluxion with IsoFlux has partnered with the Cleveland Clinic. They are aiming to improve sensitivity by timing the...

New insight into Cas9 off-target activity

LUMICKS

Jun 7, 2019

Scientists at the MRC London Institute of Medical Sciences and AstraZeneca studied how CRISPR-Cas9 differentiates...

Join the AVANCE Track & Keep your MRI on Track

Bruker Biospin

Jun 4, 2019

Your research depends on the scientific advances that you make with your MRI instrument. Take your instrument and your...

Jun 17, 2019

A novel method for isolating high-quality UHMW DNA from animal and human tissues

BioNano Genomics

May 31, 2019

New isolation protocol for isolating high-quality UHMW DNA from 10 mg of freshly frozen or liquid-preserved animal and...

ParaVision 7 software

Bruker Biospin

May 27, 2019

The preclinical MRI software ParaVision 7 delivers unsurpassed image quality and enhanced productivity for both routine...

HLS-CATCH: Cas-9 assisted gene purification

Sage Science

May 24, 2019

The CATCH process  provides researchers with the most direct access to genes for study. Target regions are defined with...

Jun 17, 2019

Introducing the first EV isolation column capable of running 100 mL samples.

IZON

May 21, 2019

As the EV field continues to grow and advance, so has the need for scalability in EV isolation. Izon is addressing this...

Show all topics (10)

Measurement of cell proliferation using NovoCyte flow cytometer

Nov 14, 2016

There are two approaches to studying cell proliferation. One is to observe changes in the cell cycle; the other is to follow the number of cell divisions over a period of time. In the first method, at the most, three cell divisions can be followed. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

Read more

Scientific paper
Application
Product news

Related technologies: Conventional flow cytometry

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey