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Sustained adhesion of T cells to APCs and formation of the immunological synapse after T cell receptor stimulation are required for the antigen-specific response. One way to measure an immunological synapse is by fluorescently label-ing the molecules that have been recruited to the synapse and imaging via confocal or conventional fluorescence microscopy. However, immunological synapses are often rare and therefore difficult to analyze objectively and statistically by traditional microscopy methods. In this study, the Amnis FlowSight imaging flow cytometry platform was employed to collect imagery of large numbers of cells to assess the percentage of T cells involved in an organized immunological synapse.
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Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.
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