Accelerate to Discover
Related topics
Registering FDG/PET mouse and AMYViD/PET-CT rat studies to brain VOI atlases
May 22, 2018
Preclinical NeuroPET is employed widely in studies of stroke, ischemia, addiction and eurodegenerative diseases. In...
Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation
May 21, 2018
Adverse tissue responses to metal wear and corrosion products from CoCr alloy implants remain a great challenge to...
New protocol: Analyze small extracellular RNAs with PippinHT
May 18, 2018
We’re pleased to see that PippinHT is featured heavily in the protocol for DNA sizing steps following PCR...
Genoox, Bionano collaborate to enhance the detection of pathogenic SVs
May 14, 2018
Bionano is now working with the global health company Genoox to develop a genome informatics workflow to integrate and...
Outstanding return on investment with the xxpress qPCR thermal cycler
May 10, 2018
Find out just how much money you could save by switching to the xxpress qPCR thermal cycler,visit the cost savings...
AVATAR Improves transfection efficiency with high viability
May 9, 2018
AVATAR lets you create any cell type even novel ones, with efficient transfection that improves reprogramming rates...
Creating a bold new future in ultrasound and photoacoustics
May 4, 2018
Fujifilm VisualSonics has been chosen as one of the top 10 visualization solution providers in the field of developing...
Bronchoscopic confocal fluorescence microscopy for in vivo assessment of free-breathing mice
May 3, 2018
Respiratory diseases with pulmonary infections resulting over 3.5 million deaths per year. Preclinical research in...
Feb 28, 2017
The authors of publication offer the MPD, a software package that automates the design of multiplex PCR primers for next-generation sequencing. The core of MPD is implemented in C for speed and uses a hashed genome to ensure primer uniqueness, avoids placing primers over sites of known variation, and efficiently pools compatible primers. A JavaScript web application (http://multiplexprimer.io) utilizing the MPD Perl package provides a convenient platform for users to make designs. Using a realistic set of genes identified by genome-wide association studies (GWAS), we achieve 90% coverage of all exonic regions using stringent design criteria. Using the first 47 primer pools for wet-lab validation, we sequenced ~25Kb at 99.7% completeness with a mean coverage of 300X among 313 samples simultaneously and identified 224 variants. The number and nature of variants we observe are consistent with high quality sequencing.
Related technologies: Target Enrichment for NGS
Get more info
Brand profile
Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.
More info at:
www.fluidigm.com/
BioFocus newsletter
Register to receive Brand new technology development, Important product updates, Interesting scientific events and more!
BioTech a.s. © 2014
Designed & developed by imagineit
Technologies
