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Travel grant ALERT! Submit ESHG abstracts today

BioNano Genomics

Feb 13, 2020

Interested in sharing your Bionano Genomics data at ESHG 2020 in Berlin? Eager to share how you have utilized the...

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COY - personalized hypoxic and anaerobic chambers since 1969

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When your research requires consistent atmospheric control to maintain anoxia, hypoxia, normoxia, or hyperoxia, you can...

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Obesity is now known to be much more than a cosmetic problem with its association with a wide plethora of diseases...

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Bionano webinar recording : EnFocus FSHD analysis

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Bionano introduced novel and reliable molecular method for facioscapulohumeral muscular dystrophy detection and fast...

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Scanned with a Bruker Skyscan-1172 high resolution microtomograph at the Department of Zoology of the University of...

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MPD: multiplex primer design for next generation targeted sequencing

Feb 28, 2017

The authors of publication offer the MPD, a software package that automates the design of multiplex PCR primers for next-generation sequencing. The core of MPD is implemented in C for speed and uses a hashed genome to ensure primer uniqueness, avoids placing primers over sites of known variation, and efficiently pools compatible primers. A JavaScript web application (http://multiplexprimer.io) utilizing the MPD Perl package provides a convenient platform for users to make designs. Using a realistic set of genes identified by genome-wide association studies (GWAS), we achieve 90% coverage of all exonic regions using stringent design criteria. Using the first 47 primer pools for wet-lab validation, we sequenced ~25Kb at 99.7% completeness with a mean coverage of 300X among 313 samples simultaneously and identified 224 variants. The number and nature of variants we observe are consistent with high quality sequencing.

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Related technologies: Target enrichment for NGS

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Fluidigm

Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

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