Accelerate to discover

Back to filter

Related topics

Travel grant ALERT! Submit ESHG abstracts today

BioNano Genomics

Feb 13, 2020

Interested in sharing your Bionano Genomics data at ESHG 2020 in Berlin? Eager to share how you have utilized the...

AutoLV analysis software

VisualSonics FujiFilm

Feb 11, 2020

AutoLV analysis software is the latest addition to VisualSonics robust suite of measurement tools customized for...

IncuCyte Live-Cell Analysis System - nearly 3,000 publications and counting!

Sartorius

Jan 21, 2020

IncuCyte has now reached over 2,000 cited publications! Search our publications filter to see what exciting research is...

Development of superparamagnetic nanoparticles as an efficient contrast agent for multimodal imaging

Bruker Biospin

Jan 16, 2020

Gadolinium‐based contrast agents are used to provide diagnostic information in clinical magnetic resonance...

Feb 18, 2020

IncuCyte live-cell analysis system for live-cell visualization of neuronal activity

Sartorius

Jan 13, 2020

A major impediment to studying diseases affecting the human nervous system is the ability to monitor, analyze, and...

Latest updates to Bionano Access and Bionano Solve

BioNano Genomics

Jan 8, 2020

Bionano Access v1.5 and Bionano Solve v3.5 are the latest updates to Bionano visualization software and analysis tools....

The qNano Gold: Obtain the most detailed Information for each individual nanoparticle

IZON

Jan 6, 2020

The qNano Gold measures particles using the Tunable Resistive Pulse Sensing (TRPS) principle. TRPS is the most powerful...

Feb 18, 2020

New analysis open modules expand capabilities of Guava Muse flow cytometer

Luminex

Dec 30, 2019

Sophisticated cell analysis doesn’t have to be complicated or costly. With the Guava Muse cell analyzer, you can now...

Show all topics (10)

Multiplex detection of rare mutations by picoliter droplet based digital PCR

Aug 29, 2016

In cancer research, the accuracy of the technology used for biomarkers detection is remarkably
important. In this context, digital PCR represents a highly sensitive and reproducible method that could serve as an appropriate tool for tumor mutational status analysis. In particular, droplet-based digital PCR approaches have been developed for detection of tumorspecific mutated alleles within plasmatic circulating DNA. Such an approach calls for the development and validation of a very significant quantity of assays, which can be extremely costly and time consuming. Herein, we evaluated assays for the detection and quantification of various mutations occurring in three genes often misregulated in cancers: the epidermal growth factor receptor (EGFR), the v-Ki-ras2 Kirsten rat sarcoma viral oncogene
homolog (KRAS) and the Tumoral protein p53 (TP53) genes. In particular, commercial competitive
allele-specific TaqMan1PCR (castPCR™) technology, as well as TaqMan1and ZEN™assays, have been evaluated for EGFR p.L858R, p.T790M, p.L861Q point mutations and in-frame deletions Del19. Specificity and sensitivity have been determined on cell lines DNA, plasmatic circulating DNA of lung cancer patients or Horizon diagnostics reference standards. To show the multiplexing capabilities of this technology, several multiplex panels for EGFR (several three- and four-plexes) have been developed, offering new"ready-to-use" tests for lung cancer patients.

Read more

Scientific paper
Product news

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

RainDance Technologies

RainDance company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases.

Related products

Capable of generating more than a billion reactions in a single day, the RainDrop system surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing.

show detail

A fast, accurate and low-cost NGS target enrichment system that features award-winning instrumentation and pre-validated gene panels, as well as flexible open source options.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey