Accelerate to discover

Back to filter

Related topics

Feel empowered with new IncuCyte SX1 live cell analysis system

Sartorius

Jul 17, 2019

Today’s new normal requires doing more with less. You need physiologically relevant, information-rich cell analysis...

Linked-read sequencing advances understanding of cancers

Sage Science

Jul 8, 2019

Using Pippin HT to filter away smaller DNA fragments maximizes the efficiency of the whole genome linked-read...

CQ1 confocal quantitative image cytometer: fighting colon cancer with killer cells

Yokogawa

Jul 2, 2019

Genetically modified immune cells can successfully destroy colon cancer cells. This has been demonstrated for the first...

Breast cancer‐derived exosomes reflect the cell of origin phenotype

IZON

Jun 24, 2019

The qNano Gold measures particles using the Tunable Resistive Pulse Sensing (TRPS) principle. TRPS is the most powerful...

Jul 24, 2019

Transcutaneous implantation of valproic acid-encapsulated

Spectral Instruments Imaging

Jun 21, 2019

The interest in alternative material systems and delivery methods for treatment of androgenetic alopecia has been...

BioFlux DCIS System, fully-integrated high content imaging under shear flow

Fluxion Biosciences

Jun 20, 2019

BioFlux DCIS is a fully-integrated high content screening platform for bridging the gap between in vitro and in vivo...

Sage products powering new methods, 2018-19

Sage Science

Jun 18, 2019

The fully automated SageELF (for Electrophoretic Lateral Fractionator) can be used to select DNA or proteins. It...

Jul 24, 2019

Low-dose imaging in a new preclinical total-body PET/CT scanner

Bruker Biospin

Jun 12, 2019

Ionizing radiation constitutes a health risk to imaging scientists and study animals. Both PET and CT produce ionizing...

Show all topics (10)

Multiplex detection of rare mutations by picoliter droplet based digital PCR

Aug 29, 2016

In cancer research, the accuracy of the technology used for biomarkers detection is remarkably
important. In this context, digital PCR represents a highly sensitive and reproducible method that could serve as an appropriate tool for tumor mutational status analysis. In particular, droplet-based digital PCR approaches have been developed for detection of tumorspecific mutated alleles within plasmatic circulating DNA. Such an approach calls for the development and validation of a very significant quantity of assays, which can be extremely costly and time consuming. Herein, we evaluated assays for the detection and quantification of various mutations occurring in three genes often misregulated in cancers: the epidermal growth factor receptor (EGFR), the v-Ki-ras2 Kirsten rat sarcoma viral oncogene
homolog (KRAS) and the Tumoral protein p53 (TP53) genes. In particular, commercial competitive
allele-specific TaqMan1PCR (castPCR™) technology, as well as TaqMan1and ZEN™assays, have been evaluated for EGFR p.L858R, p.T790M, p.L861Q point mutations and in-frame deletions Del19. Specificity and sensitivity have been determined on cell lines DNA, plasmatic circulating DNA of lung cancer patients or Horizon diagnostics reference standards. To show the multiplexing capabilities of this technology, several multiplex panels for EGFR (several three- and four-plexes) have been developed, offering new"ready-to-use" tests for lung cancer patients.

Read more

Scientific paper
Product news

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

RainDance Technologies

RainDance company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases.

Related products

Capable of generating more than a billion reactions in a single day, the RainDrop system surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing.

show detail

A fast, accurate and low-cost NGS target enrichment system that features award-winning instrumentation and pre-validated gene panels, as well as flexible open source options.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey