These applications typically require single-cell cloning to ensure that the colonies are monoclonal. With the FDA regulating clonal derivation, the method used for single-cell isolation has become important to isolate cells of interest with high fidelity and avoid using multiple cloning rounds, ensuring that a colony has a single-cell origin.
We will highlight:
-Challenges when using limiting dilution
-The importance of sheath buffer and cloning media formulation to preserve viability
-Cell line outgrowth of different sorted cells including Jurkat, HEK293, MCF-7, A549, CHO, and Chlamydomonas reinhardtii
Mar 15, 2023, 02:00 PM CEST
Dr Adonary Munoz , Associate Scientist, NanoCellect Biomedical.
Join the webinar ! be nice to your cells.