With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.
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Advances in Leukemia research using shear flow and Bioflux system
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Advance your Spatial Biology, customize your panel design with RareCyte Orion reagents
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Microfluidic cell sorter sample preparation for genomic assays
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Nov 28, 2022
Still wondering whether to join the shift to full spectrum cytometry? Easily transfer assays from your Cytek Northern...
Nanoparticle-uptake behavior in two standard cell lines, NIH/3T3 and A549
Feb 7, 2018
Uptake behavior can be precisely investigated in vitro, with sensitive high throughput methods such as flow cytometry. In this study, we investigated two different standard cell lines, human lung carcinoma (A549) and mouse fibroblast (NIH/3T3) cells, regarding their uptake behavior of titanium dioxide NPs. Cells were incubated with different concentrations of TiO2 NPs and samples were taken at certain time points to compare the uptake kinetics of both cell lines. Samples were analyzed with the help of flow cytometry by studying changes in the side and forward scattering signal. To additionally enable a detection via fluorescence, NPs were labeled with the fluorescent dye fluorescein isothiocyanate (FITC) and propidium iodide (PI). We found that NIH/3T3 cells take up the studied NPs more efficiently than A549 cells. These findings were supported by time-lapse microscopic imaging of the cells incubated with TiO2 NPs, using Etaluma Lumascope. Our results confirm that the uptake behavior of individual cell types has to be considered before interpreting any results of nanomaterial studies.
Related technologies: Live cell imaging
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