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Educational webinar: Introducing MARS Platform for gentle cell separation

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Join us for a webinar where we will introduce the new MARS Platform by Applied Cells, a...

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eSight is now powerful like never before. Learn more about new dedicated application modules for live-cell analysis: 3D...

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MARS - High Efficiency Separation of CD34+ HSC from Mobilized Blood

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Non-invasive fluorescence imaging of infiltration in a mouse model of acute lung inflammation

Nov 29, 2016

The presence of bacterial endotoxin, lipopolysaccharide (LPS) in the lung can induce a major inflammatory response leading to acute lung injury. LPS is thought to immediately activate resident macrophages, leading to the production of various cytokines, including TNF-α. This will, in turn, prompt the recruitment of inflammatory cells, mainly neutrophils, into the lung through increases in capillary permeability and alveolar edema.1 Neutrophil infiltration into the lung can be monitored by certain, activatable (smart) fluorescent probes. This study evaluates the use of an activatable optical probe that is
optically silent upon inhalation and produces a fluorescent signal only after selective cleavage by the serine protease elastase produced by neutrophils.

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