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Outstanding post-sort sample recovery on the WOLF Cell Sorter

Jun 14, 2022

Traditional droplet-based fluorescence activated cell sorters are able to isolate pure cell populations at high speeds. This often comes, however, at the cost of reduced cell recovery. Cell death induced from the high-pressure droplet-sorting mechanism, cells moving out of the droplet, and high abort rates, can all contributeto low cell recovery rates. It has been reported that traditional cell sorters can lose up to 70% of their sorted cell population. This can be a challenge when working with rare cell populations
and limited samples. Furthermore, cells adhering to the fluidics system or the collection tube, as well as post-sort centrifugation can contribute to a lower sample recovery. In contrast to traditional cell sorters, the WOLF Cell Sorter uses low pressure and gentle mechanical microfluidic sorting to efficiently isolate targeted cell populations. In this technical note, we measured the sample recovery rate from the WOLF Cell Sorter.

Learn more, be nice to your cells.

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Related technologies: Cell sorting

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Riccardo Pasculli

Head of application support

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NanoCellect

With Benchtop Microfluidic Cell Sorting, NanoCellect is committed to empowering every scientist to make discoveries one cell at a time.

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With two lasers and up to nine colors, WOLF G2 provides a gentle benchtop microfluidic cell sorting

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