Accelerate to discover

Back to filter

Related topics

Discover the Celloger live-cell analyzers : miniaturized and powerful tools for every Biologist

Curiosis

Apr 5, 2024

Celloger live-cell analyzers, image and analyze your cells in real-time, inside your incubator. With its exceptional...

The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging

Precision X-Ray

Apr 4, 2024

Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging...

A microthrombus-driven fixed-point cleaved nanosystem for preventing post-thrombolysis recurrence

IVIM Technology

Apr 3, 2024

A thrombin-responsive and fixed-point cleaved Fu@pep-CLipo was developed for highly efficient and precise thrombolysis...

Webinar: Multimodal Assessment of Hypoxia in Tumors: From the Lab to the Clinic

Bruker Biospin

Apr 2, 2024

25 April 2024, 4PM CET
This webinar will be of interest to multiple profiles in the community of biomedical
...

Apr 20, 2024

Multiplexed tissue imaging using the Orion platform to reveal the Spatial Biology of Cancer

RareCyte

Mar 27, 2024

In this webinar Prof. Sandro Santagata, will reveal how Orion high-plex imaging and the use of this data, is valuable...

A 19-color single-tube Full Spectrum Flow Cytometry for the detection of Acute Myeloid Leukemia

Cytek Biosciences

Mar 13, 2024

This recent publication in Cytometry Part A describes the development and comprehensive workflow of a single-tube,...

18F-labeled somatostatin analogs for somatostatin receptors (SSTRs) targeted PET imaging of NETs

MOLECUBES

Mar 11, 2024

A novel 18F-radiolabeled somatostatin analogue, [Al18F]NODA-MPAA-HTA, was synthesized and evaluated for positron...

Apr 20, 2024

Discover Yokogawa CellVoyager CQ1: Benchtop High-Content Analysis System

Yokogawa

Mar 8, 2024

Unlike flow cytometers, the CellVoyager CQ1 confocal quantitative image cytometer does not require pretreatment such as...

Show all topics (10)

Quantification of cell migration and invasion using the IncuCyte chemotaxis assay

Mar 15, 2016

Effective in vitro models of directed cell migration, or chemotaxis, are necessary for both basic research and drug discovery. This application note describes a new methodology for studying chemotactic cell migration and invasion using the IncuCyte ZOOM Live-Cell Imaging system. This novel approach enables real-time visualization and automated analysis of chemotactic migration and invasion in a 96-well format within a tissue culture incubator.

By incorporating a made-for-purpose, optically clear membrane into a transmembrane geometry, the IncuCyte chemotaxis assay facilitates the label-free visualization and quantitation of directed cell migration and invasion. The design of the IncuCyte ClearView 96-Well Cell Migration Plate ensures a stable gradient over 72 hours and requires only 1,000–5,000 cells per well. In contrast to traditional Boyden-chamber methodologies, cells in the IncuCyte chemotaxis assay are required to migrate on a biologically relevant surface toward the chemoattractant. The result is 96 wells of reproducible kinetic data supported by images and movies, requiring very few cells and minimal hands-on time.

 

Read the application note.

Scientific paper
Application
Video

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey