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Bionano fall user workshop

BioNano Genomics

May 23, 2018

We are happy to announce the Bionano Genomics’ 2018 user workshop taking place in San Diego on October 15-16,...

Registering FDG/PET mouse and AMYViD/PET-CT rat studies to brain VOI atlases

Bruker Biospin

May 22, 2018

Preclinical NeuroPET is employed widely in studies of stroke, ischemia, addiction and eurodegenerative diseases. In...

Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation

Etaluma

May 21, 2018

Adverse tissue responses to metal wear and corrosion products from CoCr alloy implants remain a great challenge to...

New protocol: Analyze small extracellular RNAs with PippinHT

Sage Science

May 18, 2018

We’re pleased to see that PippinHT is featured heavily in the protocol for DNA sizing steps following PCR...

May 23, 2018

Genoox, Bionano collaborate to enhance the detection of pathogenic SVs

BioNano Genomics

May 14, 2018

Bionano is now working with the global health company Genoox to develop a genome informatics workflow to integrate and...

Outstanding return on investment with the xxpress qPCR thermal cycler

BJS Biotechnologies

May 10, 2018

Find out just how much money you could save by switching to the xxpress qPCR thermal cycler,visit the cost savings...

AVATAR Improves transfection efficiency with high viability

Xcell Biosciences

May 9, 2018

AVATAR lets you create any cell type even novel ones, with efficient transfection that improves reprogramming rates...

May 23, 2018

Creating a bold new future in ultrasound and photoacoustics

VisualSonics FujiFilm

May 4, 2018

Fujifilm VisualSonics has been chosen as one of the top 10 visualization solution providers in the field of developing...

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Quantifying the impact of a CD19-BiTE combining flow cytometry & real-time cell analysis

Jun 9, 2017

The ability to quantitatively correlate T cell biomarker expression/secretion with  target cell killing is critical in both basic and applied studies of tumor immunology. Coupling flow cytometry with multiplex bead-based immunoassays enables quantitative measurement of multiple secreted targets in a single sample simultaneously. In this Application Note, we demonstrate a workflow involving the use of an xCELLigence Real-Time Cell Analysis (RTCA) instrument in conjunction with a NovoCyte flow cytometer to study both CTL-mediated destruction of tumor cells and the corresponding secretion of cytokines and cytolytic proteins by CTLs. Representing a promising new class of therapeutics, bispecific T-cell engagers (BiTEs) harness the power of the adaptive immune response by enhancing the ability of CTLs to specifically recognize and eliminate tumors. For example, CD19-BiTEs  are designed to simultaneously bind CD3 on CTLs and CD19 on cells of B cell lineage, thereby enhancing the efficiency of CTL effector functions against various B cell derived tumors. Herein, the enhancement effects of CD19-BiTEs have been evaluated using three distinct assays. While target cell death was monitored using an RTCA impedance assay and a flow cytometry-based cytolysis assay, secretion of cytokines and cytolytic proteins was quantified in a bead-based multiplex flow cytometry assay.

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Related technologies: Conventional Flow Cytometry

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Riccardo Pasculli

Field Application Specialist

Riccardo

Pasculli

+420 731 127 717

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Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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