Accelerate to discover

Back to filter

Related topics

High-frequency Ultrasound System For Preclinical Imaging

S-Sharp

May 13, 2024

The Prospect T1 is an innovative high-frequency ultrasound system designed specifically for in vivo preclinical imaging...

Automatically Acquire Bioluminescent Kinetic Curve Data in Real Time

Spectral Instruments Imaging

May 10, 2024

New Kinetics Mode of Aura software ensures you're capturing peak data for optimal sensitivity & reproducibility

“Range+T “ for Tight Sizing of HMW Libraries

Sage Science

May 10, 2024

We decided to do a deep dive into Range+T to get a better handle the method, and to develop best practices for using...

SteriRadHD—The Next Generation Cannabis Anti-Microbial Remediation System

Precision X-Ray

May 9, 2024

Precision X-Ray, Inc. Unveils SteriRadHD—The Next Generation Cannabis Anti-Microbial Remediation System. SteriRadHD...

May 25, 2024

April 2024 publication revealing benefits of using intravital microscopy in trascriptomics studies

IVIM Technology

May 8, 2024

Transcriptional activation of Bmal1 drives the inflammatory activity of monocytes by modulating mitochondrial unfolded...

Minimal information for studies of extracellular vesicles (MISEV2023)

IZON

May 7, 2024

MISEV2023 is intended to aid any and all EV researchers: from those just starting their EV journey to more established...

Omics Studies of Tumor Cells under Microgravity Conditions

CelVivo

May 6, 2024

It is important for the analysis of data from space experiments to distinguish unspecific stress reactions from...

May 25, 2024

Column-Free CD14+ Monocyte Isolation using 50nm Superparamagentic Beads on MARS® Bar

Applied Cells

Apr 25, 2024

The MARS® Bar Magnetic Separation Platform is a closed and automated isolation for cell therapy development and...

Show all topics (10)

Quantifying the impact of a CD19-BiTE combining flow cytometry & real-time cell analysis

Jun 9, 2017

The ability to quantitatively correlate T cell biomarker expression/secretion with  target cell killing is critical in both basic and applied studies of tumor immunology. Coupling flow cytometry with multiplex bead-based immunoassays enables quantitative measurement of multiple secreted targets in a single sample simultaneously. In this Application Note, we demonstrate a workflow involving the use of an xCELLigence Real-Time Cell Analysis (RTCA) instrument in conjunction with a NovoCyte flow cytometer to study both CTL-mediated destruction of tumor cells and the corresponding secretion of cytokines and cytolytic proteins by CTLs. Representing a promising new class of therapeutics, bispecific T-cell engagers (BiTEs) harness the power of the adaptive immune response by enhancing the ability of CTLs to specifically recognize and eliminate tumors. For example, CD19-BiTEs  are designed to simultaneously bind CD3 on CTLs and CD19 on cells of B cell lineage, thereby enhancing the efficiency of CTL effector functions against various B cell derived tumors. Herein, the enhancement effects of CD19-BiTEs have been evaluated using three distinct assays. While target cell death was monitored using an RTCA impedance assay and a flow cytometry-based cytolysis assay, secretion of cytokines and cytolytic proteins was quantified in a bead-based multiplex flow cytometry assay.

Read more

Scientific paper
Application
Product news

Related technologies: Conventional flow cytometry

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey