Accelerate to discover

Back to filter

Related topics

High-frequency Ultrasound System For Preclinical Imaging

S-Sharp

May 13, 2024

The Prospect T1 is an innovative high-frequency ultrasound system designed specifically for in vivo preclinical imaging...

“Range+T “ for Tight Sizing of HMW Libraries

Sage Science

May 10, 2024

We decided to do a deep dive into Range+T to get a better handle the method, and to develop best practices for using...

April 2024 publication revealing benefits of using intravital microscopy in trascriptomics studies

IVIM Technology

May 8, 2024

Transcriptional activation of Bmal1 drives the inflammatory activity of monocytes by modulating mitochondrial unfolded...

Minimal information for studies of extracellular vesicles (MISEV2023)

IZON

May 7, 2024

MISEV2023 is intended to aid any and all EV researchers: from those just starting their EV journey to more established...

May 24, 2024

Omics Studies of Tumor Cells under Microgravity Conditions

CelVivo

May 6, 2024

It is important for the analysis of data from space experiments to distinguish unspecific stress reactions from...

Column-Free CD14+ Monocyte Isolation using 50nm Superparamagentic Beads on MARS® Bar

Applied Cells

Apr 25, 2024

The MARS® Bar Magnetic Separation Platform is a closed and automated isolation for cell therapy development and...

Spatial and temporal profiling of the complement system uncovered novel functions of the alternative

Vizgen

Apr 24, 2024

Mounting evidence implicated the classical complement pathway (CP) in normal brain development, and the pathogenesis of...

May 24, 2024

The Cytek "Spectral Duo": the first and only Spectral Cell Sorter and Analyzer made for each other

Cytek Biosciences

Apr 24, 2024

The Cytek Aurora CS system is the only cell sorter on the market with a matched full spectrum analyzer. All the...

Show all topics (10)

Real-time and quantitative analysis of Macrophage Phagocytosis with RTCA eSight

Feb 23, 2024

Considering the critical roles that phagocytosis plays in microbial defense, wound healing, development,
and tissue homeostasis, it is not surprising that numerous assays have been developed to study phagocytosis in vitro. The primary legacy assays involve microscopy, flow cytometry, or spectrophotometry/spectrofluorimetry. In these assays phagocytes are typically incubated for a fixed period with particles (dextran, zymosan, E. coli) that are labeled to be constitutively fluorescent, followed by endpoint analysis. Quantification of phagocytosis by microscopy often requires cell fixation followed by manually counting the number of particles inside each cell, which is tedious and not amenable to
high throughput studies. Quantification of phagocytosis by spectrofluorimetry requires that uningested particles either be quenched or physically removed before analysis. Variations on these
assays, where particles are coupled to enzymes that are detectable using colorimetric substrates once cells have been fixed and permeabilized, still require a significant amount of hands-on time. The same is also true of flow cytometry‑based assays where phagocytes must be collected from the well bottom before they can be analyzed. In contrast to all these legacy methods, the eSight assay described here only requires mixing phagocytes with substrates; zero handing steps are subsequently required. Another distinguishing feature of this eSight phagocytosis assay is its continuous nature. Tracking phagocytosis in real time makes it possible to focus analyses on the most appropriate time regions.

Read more

 

Technical breakthrough
Application

Related technologies: Live cell imaging

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The eSight system combines the label-free xCELLigence RTCA technology with live cell imaging in 3 colors (red, green, and blue), all inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey