Accelerate to discover

Back to filter

Related topics

Structural variants identified by Oxford Nanopore PromethION sequencing of the human genome

Sage Science

Aug 16, 2019

The BluePippin was used for High Pass DNA size selection prior to library construction. ONT Promethion was used for...

Fluxion Biosciences launches custom liquid biopsy NGS panel development

Fluxion Biosciences

Aug 14, 2019

Fluxion a leading developer of precision solutions for life science research and diagnostics, announced that can...

Superior flow cytometry data with NEW Curiox Laminar Wash systems - centrifuge - less washing

Curiox

Aug 12, 2019

The Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry....

Electroporation using Lumascope: A sustainable method for Adipogenous Mesenchymal Stem Cells

Etaluma

Aug 7, 2019

Human mesenchymal stem cells derived from adipose tissue (AD-hMSCs) represent a promising source for tissue engineering...

Aug 19, 2019

The best way to fragment DNA for genomic and paired end library construction

Digilab

Aug 5, 2019

Digilab NextGen Shear DNA Shearing offers the simplest, most reproducible, and most controllable method available for...

New Cytek Full Spectrum Viewer tool, a powerful guide for your fluorochrome choices

Cytek Biosciences

Aug 2, 2019

Want to see which fluorochromes have unique signatures and which are identical? Then the Cytek Full Spectrum Viewer...

Software Update: Bionano releases a new pipeline for low Aalelic fraction SVs

BioNano Genomics

Jul 31, 2019

Bionano Solve v3.4 is a major update that introduces an entirely new pipeline to detect SVs at low allelic fraction and...

Aug 19, 2019

On-cartridge preparation and evaluation of 68Ga-, 89Zr- and 64Cu-precursors for cell radiolabelling

Bruker Biospin

Jul 29, 2019

Method of on-cartridge formation of gallium-68, zirconium-89 and copper-64 complexes in small volumes suitable for cell...

Show all topics (10)

Real-time quantitative analysis of apoptosis

Jun 21, 2016

Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. Aberrations in apoptotic signaling are implicated in a range of human pathologies including cancer, autoimmune disease and neurodegeneration. Induction of apoptosis leads, in most cases, to the activation of caspases (cysteinyl aspartate proteinases) and plasma membrane alterations. The activation of caspase-3 or caspase-7 results in the irreversible commitment of the cell to apoptotic death, and is considered a reliable marker for apoptosis. The regulated loss of plasma membrane phosphatidylserine (PS) symmetry is also a classical marker of apoptosis. Dying cells trigger the translocation of the normally inward-facing PS to the cellular surface, allowing for early phagocytic recognition of the dying cell by surrounding phagocytes.

The IncuCyte Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the IncuCyte ZOOM live cell analysis system and employ simple mix-and-read protocols. Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the IncuCyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the IncuCyte ZOOM integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.

IncuCyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.

Application
Product news

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey