Accelerate to discover

Back to filter

Related topics

Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

ACCELA educational webinar: Unlocking Spatial Biology with RareCyte Orion

RareCyte

Mar 16, 2023

Experience Spatial Biology with Orion Multiplex Imaging. Orion is a benchtop, high...

Recorded webinar: Anesthesia Considerations in Small Animal Imaging

Spectral Instruments Imaging

Mar 13, 2023

Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...

Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay

Cytek Biosciences

Mar 13, 2023

This kit has been designed to enumerate all major leukocyte subsets and it mirrors and expands on those identified in a...

Mar 25, 2023

Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II

RareCyte

Mar 7, 2023

CyteFinder II Instruments are high speed, whole slide imaging systems with options for liquid biopsy analysis and...

NanoCellect Webinar: Optimizing the Cell Line Development Process with Microfluidic Cell Sorting

NanoCellect

Mar 3, 2023

Single-cell selection and cloning are required for bioengineering workflows such as antibody production, cell therapy,...

New Kit for Bacterial analysis expands capability of Guava Muse flow cytometer

Luminex

Feb 20, 2023

With the Guava Muse cell analyzer, you can now achieve highly quantitative results at a fraction of the price, effort,...

Mar 25, 2023

Flash news - did you know, that you can use Singulator S100 also for plant research?

S2 Genomics

Feb 10, 2023

In this short TechNote you can find how Singulator can help the data quality in Single cell sequencing - now even in...

Show all topics (10)

Real-time quantitative analysis of apoptosis

Jun 21, 2016

Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. Aberrations in apoptotic signaling are implicated in a range of human pathologies including cancer, autoimmune disease and neurodegeneration. Induction of apoptosis leads, in most cases, to the activation of caspases (cysteinyl aspartate proteinases) and plasma membrane alterations. The activation of caspase-3 or caspase-7 results in the irreversible commitment of the cell to apoptotic death, and is considered a reliable marker for apoptosis. The regulated loss of plasma membrane phosphatidylserine (PS) symmetry is also a classical marker of apoptosis. Dying cells trigger the translocation of the normally inward-facing PS to the cellular surface, allowing for early phagocytic recognition of the dying cell by surrounding phagocytes.

The IncuCyte Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the IncuCyte ZOOM live cell analysis system and employ simple mix-and-read protocols. Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the IncuCyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the IncuCyte ZOOM integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.

IncuCyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.

Application
Product news

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey