Accelerate to discover

Back to filter

Related topics

Download today your IsoPlexis e-book: understanding immune Cell Function In Cancer

IsoPlexis

Dec 1, 2021

Understanding complex immune-cancer interactions is critical to identifying therapeutic targets, devising novel avenues...

IsoPlexis technology: accelerating decisions in immunotherapy with predictive single-cell response

IsoPlexis

Nov 25, 2021

The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell...

Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer

Agilent technologies

Nov 23, 2021

Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...

Jan 18, 2022

Discover the advanced Detector Technology in Agilent NovoCyte flow cytometers

Agilent technologies

Nov 8, 2021

This technical overview describes advancements in detector technology, and highlights examples where optimal signal...

Introducing isoLight system - Automated Cellular Proteomics hub

IsoPlexis

Nov 4, 2021

The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell...

Jan 18, 2022

Introducing Cytek NEW 25-Color Immunoprofiling Assay & Kit!

Cytek Biosciences

Oct 25, 2021

To unleash the power of the Cytek full spectrum cytometry system, Cytek scientists designed and developed the 25-Color...

Show all topics (10)

Real-time quantitative analysis of apoptosis

Jun 21, 2016

Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. Aberrations in apoptotic signaling are implicated in a range of human pathologies including cancer, autoimmune disease and neurodegeneration. Induction of apoptosis leads, in most cases, to the activation of caspases (cysteinyl aspartate proteinases) and plasma membrane alterations. The activation of caspase-3 or caspase-7 results in the irreversible commitment of the cell to apoptotic death, and is considered a reliable marker for apoptosis. The regulated loss of plasma membrane phosphatidylserine (PS) symmetry is also a classical marker of apoptosis. Dying cells trigger the translocation of the normally inward-facing PS to the cellular surface, allowing for early phagocytic recognition of the dying cell by surrounding phagocytes.

The IncuCyte Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the IncuCyte ZOOM live cell analysis system and employ simple mix-and-read protocols. Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the IncuCyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the IncuCyte ZOOM integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.

IncuCyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.

Application
Product news

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey