Accelerate to discover

Back to filter

Related topics

Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

ACCELA educational webinar: Unlocking Spatial Biology with RareCyte Orion

RareCyte

Mar 16, 2023

Experience Spatial Biology with Orion Multiplex Imaging. Orion is a benchtop, high...

Recorded webinar: Anesthesia Considerations in Small Animal Imaging

Spectral Instruments Imaging

Mar 13, 2023

Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...

Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay

Cytek Biosciences

Mar 13, 2023

This kit has been designed to enumerate all major leukocyte subsets and it mirrors and expands on those identified in a...

Mar 25, 2023

Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II

RareCyte

Mar 7, 2023

CyteFinder II Instruments are high speed, whole slide imaging systems with options for liquid biopsy analysis and...

NanoCellect Webinar: Optimizing the Cell Line Development Process with Microfluidic Cell Sorting

NanoCellect

Mar 3, 2023

Single-cell selection and cloning are required for bioengineering workflows such as antibody production, cell therapy,...

New Kit for Bacterial analysis expands capability of Guava Muse flow cytometer

Luminex

Feb 20, 2023

With the Guava Muse cell analyzer, you can now achieve highly quantitative results at a fraction of the price, effort,...

Mar 25, 2023

Flash news - did you know, that you can use Singulator S100 also for plant research?

S2 Genomics

Feb 10, 2023

In this short TechNote you can find how Singulator can help the data quality in Single cell sequencing - now even in...

Show all topics (10)

Scaling to singles - summary of single cell mRNAseq methods

Jan 20, 2017

Fluidigm’s C1 system is encased in a benchtop machine that automatically orchestrates the steps of Smart-seq, taking your cell suspension and isolating and lysing cells, reverse transcribing their mRNA, and amplifying the resulting cDNA. It requires nonreusable microfluidic array chips that analyze 96 cells. Amplified cDNA is then sequenced or detected using qPCR. Switching mechanism at 5′ end of the RNA transcript (Smart-seq) is one of the only sequencing protocols that allows you to generate full-length coverage of transcriptomes from single cells, which is important if you’re studying allele-specific gene expression or splice variants.

Read more

Scientific paper
Technical breakthrough
Application

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

Fluidigm

Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

Related products

The first automated solution for single-cell genomics, now capable of even more

show detail

The system for precise manipulation of biological pathways for in vitro cell culture.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey