Accelerate to discover

Back to filter

Related topics

X-ray-virus-inactivation

Precision X-Ray

Oct 20, 2021

Ionizing radiation approaches are effective but use of radioactive radioisotopic elements have become difficult to...

Duke uses XRAD 225 CX to test radiosensitivity hypothesis

Precision X-Ray

Oct 18, 2021

Image guided radiotherapy in a preclinical setting couldn’t be more useful than the treatment of brain tumors, where...

Curiox webinar: Laminar Wash enables single-cell multiomics and cell hashing

Curiox

Oct 11, 2021

Professor Mats Bemark’s lab at the University of Gothenburg in Sweden studies how the immune system responds to...

Falling in love with Magnetic resonance

Bruker Biospin

Oct 4, 2021

Magnetic Resonance (MR) is more than just analytical science, it is unique, it is a passion that won’t let you go

Oct 25, 2021

The roles of preclinical optical imaging in cancer research

Spectral Instruments Imaging

Sep 22, 2021

Watch the last Spectral Instrument Imaging webinar with Dr. Michael Henry who will shares key data sets from his work...

See What's Possible with the PippinHT

Sage Science

Sep 14, 2021

Programmable DNA size selection enables new discoveries by helping optimize the library detected by a sequencer. That’s...

Magnetic resonance elastography techniques and preclinical applications

Bruker Biospin

Sep 14, 2021

It has been known for centuries that tissue stiffness can change in the presence of disease, but quantifying such...

Oct 25, 2021

Ex Vivo dental imaging is directly translatable to the clinic

Bruker Biospin

Sep 14, 2021

Although patients are too large in size for preclinical microCT scanners, it still has a big significance in clinical...

Show all topics (10)

Spectroscopic photoacoustic molecular imaging of breast cancer using a B7-H3-targeted ICG contrast

Jun 5, 2017

Breast cancer imaging methods lack diagnostic accuracy, in particular for patients with dense breast tissue, and improved techniques are critically needed. The purpose of this study was to evaluate antibody-indocyanine green (ICG) conjugates, which undergo dynamic absorption spectrum shifts after cellular endocytosis and degradation, and spectroscopic photoacoustic (sPA) imaging to differentiate normal breast tissue from breast cancer by imaging B7-H3, a novel breast cancer associated molecular target.
Quantitative immunohistochemical staining of endothelial and epithelial B7-H3 expression was assessed in 279 human breast tissue samples, including normal (n=53), benign lesions (11 subtypes, n=129), and breast cancers (4 subtypes, n=97). After absorption spectra of intracellular and degraded B7-H3-ICG and Isotype control-ICG (Iso-ICG) were characterized, sPA imaging in a transgenic murine breast cancer model (FVB/N-Tg(MMTVPyMT)634Mul) was performed and compared to imaging of control conditions [B7-H3-ICG in tumor negative animals (n=60), Iso-ICG (n=30), blocking B7-H3+B7-H3-ICG (n=20), and free ICG (n=20)] and validated with ex vivo histological analysis.
Immunostaining showed differential B7-H3 expression on both the endothelium and tumor epithelium in human breast cancer with an area under the ROC curve of 0.93 to differentiate breast cancer vs non-cancer. Combined in vitro/in vivo imaging showed that sPA allowed specific B7-H3-ICG detection down to the 13 nM concentration and differentiation from Iso-ICG. sPA molecular imaging of B7-H3-ICG showed a 3.01-fold (P<0.01) increase in molecular B7-H3-ICG signal in tumors compared to control conditions.
B7-H3 is a promising target for both vascular and epithelial sPA imaging of breast cancer. Leveraging antibody-ICG contrast agents and their dynamic optical absorption spectra allows for highly specific sPA imaging of breast cancer.

Read more

Scientific paper
Application

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 255 700 886

Send Message

Brand profile

VisualSonics FujiFilm

FujiFilm VisualSonics Inc. is a manufacturer of real-time, in vivo, high-resolution micro-imaging systems designed specifically for preclinical research.

Related products

Photoacoustics is a ground-breaking, real time imaging modality for visualizing small animal anatomy and analyzing function in vivo and non-invasively

show detail

Vevo LAZR-X combines high frequency ultrasound and photoacoustics into one platform for high resolution anatomical, functional and molecular imaging

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey