Accelerate to discover

Back to filter

Related topics

Squaraine fluorophore as a NIR-fluorescent probe for the in vivo detection of diagnostic enzymes

Spectral Instruments Imaging

Nov 19, 2018

An oligo(ethylene glycol)-functionalized squaraine fluorophore has been developed as an NIR-fluorescent probe that can...

IncuCyte neuronal activity assay

Sartorius

Nov 13, 2018

The generation and maturation of neuronal networks are necessary characteristics of neurogenesis, providing...

The most advanced software for preclinical imaging research

Bruker Biospin

Nov 7, 2018

Modern imaging laboratories hosting PET/MR instruments profit from the fully integrated common imaging platform...

IZON qEV - Isolate your exosomes in just 15 minutes

IZON

Oct 25, 2018

Extracellular  vesicles  (EVs)  are  prevalent  in biological  fluids  and  are  of  great  interest  to researchers ...

Nov 19, 2018

COY - personalized hypoxic chambers

COY laboratory products

Oct 18, 2018

When your research requires consistent atmospheric control to maintain hypoxia, normoxia, or hyperoxia, you can count...

Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs

Fluxion Biosciences

Oct 12, 2018

Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...

Target large genomic regions with 10x genomics + SageHLS

Sage Science

Oct 9, 2018

With customized Cas9 nucleases, scientists can now isolate specific large genomic DNA fragments with the SageHLS...

Nov 19, 2018

Coy anaerobic chambers #1 choice in microbiology

COY laboratory products

Oct 5, 2018

Used for over 40 years in anaerobic microbiology research, COY chambers are also critical to many other research areas...

Show all topics (10)

Spectroscopic photoacoustic molecular imaging of breast cancer using a B7-H3-targeted ICG contrast

Jun 5, 2017

Breast cancer imaging methods lack diagnostic accuracy, in particular for patients with dense breast tissue, and improved techniques are critically needed. The purpose of this study was to evaluate antibody-indocyanine green (ICG) conjugates, which undergo dynamic absorption spectrum shifts after cellular endocytosis and degradation, and spectroscopic photoacoustic (sPA) imaging to differentiate normal breast tissue from breast cancer by imaging B7-H3, a novel breast cancer associated molecular target.
Quantitative immunohistochemical staining of endothelial and epithelial B7-H3 expression was assessed in 279 human breast tissue samples, including normal (n=53), benign lesions (11 subtypes, n=129), and breast cancers (4 subtypes, n=97). After absorption spectra of intracellular and degraded B7-H3-ICG and Isotype control-ICG (Iso-ICG) were characterized, sPA imaging in a transgenic murine breast cancer model (FVB/N-Tg(MMTVPyMT)634Mul) was performed and compared to imaging of control conditions [B7-H3-ICG in tumor negative animals (n=60), Iso-ICG (n=30), blocking B7-H3+B7-H3-ICG (n=20), and free ICG (n=20)] and validated with ex vivo histological analysis.
Immunostaining showed differential B7-H3 expression on both the endothelium and tumor epithelium in human breast cancer with an area under the ROC curve of 0.93 to differentiate breast cancer vs non-cancer. Combined in vitro/in vivo imaging showed that sPA allowed specific B7-H3-ICG detection down to the 13 nM concentration and differentiation from Iso-ICG. sPA molecular imaging of B7-H3-ICG showed a 3.01-fold (P<0.01) increase in molecular B7-H3-ICG signal in tumors compared to control conditions.
B7-H3 is a promising target for both vascular and epithelial sPA imaging of breast cancer. Leveraging antibody-ICG contrast agents and their dynamic optical absorption spectra allows for highly specific sPA imaging of breast cancer.

Read more

Scientific paper
Application

Related technologies: Photoacoustic imaging

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 737 202 127

Send Message

Brand profile

VisualSonics FujiFilm

FujiFilm VisualSonics Inc. is a manufacturer of real-time, in vivo, high-resolution micro-imaging systems designed specifically for preclinical research.

Related products

Photoacoustics is a ground-breaking, real time imaging modality for visualizing small animal anatomy and analyzing function in vivo and non-invasively

show detail

Vevo LAZR-X combines high frequency ultrasound and photoacoustics into one platform for high resolution anatomical, functional and molecular imaging

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey