Accelerate to discover

Back to filter

Related topics

Discover the newly re-designed TissueGnostics portfolio

TissueGnostics

Sep 18, 2020

TissueGnostics is a solution provider for Precision Medicine and provides fully integrated cutting-edge tissue...

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

Are you still using traditional methodologies for your Oncology cell metabolism studies?

Sartorius

Sep 10, 2020

In this infographic, learn about the challenges experienced by scientists in analysing cellular metabolism and how...

Using DNA repair and machine learning to improve cancer risk prediction

Luminex

Sep 7, 2020

Across the world, breast cancer continues to be the leading type of cancer in women, accounting for 25% of all cases....

Sep 21, 2020

Quantitative evaluation of pulmonary function with hyperpolarized xenon gas MR

Bruker Biospin

Aug 20, 2020

Hyperpolarized 129Xe MR has been proven to be a powerful tool to evaluate the pulmonary function and has been widely...

Get more out of your full spectrum panel with Cytek’s new reagent family

Cytek Biosciences

Aug 17, 2020

Cytek Biosciences has already redefined what is possible in flow cytometry. The new Cytek cFluor family of reagents...

Applications of Hyperpolarized 129Xe MRI for functional imaging of the lungs

Bruker Biospin

Aug 11, 2020

2015 marked the first ever translation of hyperpolarized gas MRI to diagnostic clinical practice. Research at the...

Sep 21, 2020

Detection of Rare Cells and Circulating Tumor Cells using NovoCyte flow cytometer

Acea Biosciences

Aug 11, 2020

Circulating tumor cells (CTC) are rare cells shed from a solid tumor or metastases that circulate through the...

Show all topics (10)

Stem cell differentiation is tuned by oxygen concentration and pressure

Nov 29, 2017

While extensive research has focused on soluble factors to optimize stem cell culture, conditions such as hypoxia, atmospheric pressure, and the composition and organization of the extracellular matrix are also important drivers of stem cell differentiation and cell function. However, no study to date has systematically analyzed the contribution of these factors in the maintenance and differentiation of stem cells, leading to uncertainty surrounding the extracellular factors that dictate stem cell state.To address this, Xcell Biosciences has developed a novel stem cell culturing platform, the Avatar SystemTM, which allows for tunable control of the microenvironment and uniquely offers customizable settings for oxygen and hydrostatic pressure. In a recent study, Xcell scientists analyzed human pluripotent stem cells to characterize their underlying biology and to demonstrate the utility of the Avatar system. Cells were cultured in minimally supportive media to allow stem cell state to drift. The Avatar system was used to tune environmental conditions and determine the impact of oxygen tension and pressure in guiding stem cell fate.

For this project, scientists reprogrammed primary human dermal fibroblasts via episomal expression of key stemness factors (Sox2, Nanog, and Oct4) while cultured in altered oxygen concentration (1% – 5%) and atmospheric pressure (0 PSI – 5 PSI) using the Avatar system compared to conventional culture methods. Having generated iPSCs, scientists next aimed to assess their pluripotent potential relative to the condition in which reprogramming and subsequent long term culture was performed.

Read more

Scientific paper
Technical breakthrough
Application

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey