Accelerate to discover

Back to filter

Related topics

Discover the newly re-designed TissueGnostics portfolio

TissueGnostics

Sep 18, 2020

TissueGnostics is a solution provider for Precision Medicine and provides fully integrated cutting-edge tissue...

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

Get more out of your full spectrum panel with Cytek’s new reagent family

Cytek Biosciences

Aug 17, 2020

Cytek Biosciences has already redefined what is possible in flow cytometry. The new Cytek cFluor family of reagents...

Introducing Amnis AI Image Analysis software

Luminex

Aug 4, 2020

Amnis AI Image Analysis Software combines the power of high dimensional data reduction with artificial intelligence,...

Sep 22, 2020

The Role of Micro-CT in Imaging Breast Cancer Specimens

Bruker Biospin

Jun 17, 2020

The goal of breast cancer surgery is to remove all of the cancer with a minimum of normal tissue, but absence of full...

Cytek Northern Lights: 41 parameters with 3 lasers only

Cytek Biosciences

Jun 12, 2020

Cytek Biosciences Northern Lights incorporates the same innovations as the Aurora, but at a lower price point. It...

Bruker´s micro-CT used for some of the World’s First in vivo COVID-19 Imaging Experiments

Bruker Biospin

Jun 9, 2020

Bruker's BioSpin PreClinical Imaging Division reports the use of the Bruker SkyScan 1278 in vivo micro-CT system at the...

Sep 22, 2020

A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Spectral Instruments Imaging

Jun 3, 2020

Soft tissue sarcomas pose a significant clinical challenge, with approximately 12,000 new diagnoses and an estimated...

Show all topics (10)

The Access Array system: indispensable support tool for NGS

Oct 18, 2016

With the increasing democratization of high-throughput sequencing (HTS) technologies, along with the concomitant increase in sequence yield per dollar, many researchers are exploring HTS for microbial community ecology. Many elements of experimental design can drastically affect the final observed community structure, notably the choice of primers for amplification prior to sequencing. Some targeted microbes can fail to amplify due to primer-targeted sequence divergence and be omitted from obtained sequences, leading to differences among primer pairs in the sequenced organisms even when targeting the same community. This potential source of taxonomic bias in HTS makes it prudent to investigate how primer choice will affect the sequenced community prior to investing in a costly community-wide sequencing effort. Here, we use Fluidigm's microfluidic Access Arrays (IFC) followed by Illumina MiSeq Nano sequencing on a culture-derived local mock community to demonstrate how this approach allows for a low-cost combinatorial investigation of primer pairs and experimental samples (up to 48 primer pairs and 48 samples) to determine the most effective primers that maximize obtained communities whilst minimizing taxonomic biases.

Read more

Scientific paper
Product news

Related technologies: Target enrichment for NGS

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

Fluidigm

Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

Related products

Superior library preparation on the widest scale with a streamlined workflow and low costs.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey