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Bionano fall user workshop

BioNano Genomics

May 23, 2018

We are happy to announce the Bionano Genomics’ 2018 user workshop taking place in San Diego on October 15-16,...

Registering FDG/PET mouse and AMYViD/PET-CT rat studies to brain VOI atlases

Bruker Biospin

May 22, 2018

Preclinical NeuroPET is employed widely in studies of stroke, ischemia, addiction and eurodegenerative diseases. In...

Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation

Etaluma

May 21, 2018

Adverse tissue responses to metal wear and corrosion products from CoCr alloy implants remain a great challenge to...

New protocol: Analyze small extracellular RNAs with PippinHT

Sage Science

May 18, 2018

We’re pleased to see that PippinHT is featured heavily in the protocol for DNA sizing steps following PCR...

May 23, 2018

Genoox, Bionano collaborate to enhance the detection of pathogenic SVs

BioNano Genomics

May 14, 2018

Bionano is now working with the global health company Genoox to develop a genome informatics workflow to integrate and...

Outstanding return on investment with the xxpress qPCR thermal cycler

BJS Biotechnologies

May 10, 2018

Find out just how much money you could save by switching to the xxpress qPCR thermal cycler,visit the cost savings...

AVATAR Improves transfection efficiency with high viability

Xcell Biosciences

May 9, 2018

AVATAR lets you create any cell type even novel ones, with efficient transfection that improves reprogramming rates...

May 23, 2018

Creating a bold new future in ultrasound and photoacoustics

VisualSonics FujiFilm

May 4, 2018

Fujifilm VisualSonics has been chosen as one of the top 10 visualization solution providers in the field of developing...

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The Access Array system: indispensable support tool for NGS

Oct 18, 2016

With the increasing democratization of high-throughput sequencing (HTS) technologies, along with the concomitant increase in sequence yield per dollar, many researchers are exploring HTS for microbial community ecology. Many elements of experimental design can drastically affect the final observed community structure, notably the choice of primers for amplification prior to sequencing. Some targeted microbes can fail to amplify due to primer-targeted sequence divergence and be omitted from obtained sequences, leading to differences among primer pairs in the sequenced organisms even when targeting the same community. This potential source of taxonomic bias in HTS makes it prudent to investigate how primer choice will affect the sequenced community prior to investing in a costly community-wide sequencing effort. Here, we use Fluidigm's microfluidic Access Arrays (IFC) followed by Illumina MiSeq Nano sequencing on a culture-derived local mock community to demonstrate how this approach allows for a low-cost combinatorial investigation of primer pairs and experimental samples (up to 48 primer pairs and 48 samples) to determine the most effective primers that maximize obtained communities whilst minimizing taxonomic biases.

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Related technologies: Target Enrichment for NGS

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Regina Fillerová

Field Application Specialist

Regina

Fillerová

+420 731 127 718

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Fluidigm

Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

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