Accelerate to discover

Back to filter

Related topics

Micro-ct study of the anatomy of a grasshopper

Bruker Biospin

Jan 23, 2020

Scanned with a Bruker Skyscan-1172 high resolution microtomograph at the Department of Zoology of the University of...

IncuCyte Live-Cell Analysis System - nearly 3,000 publications and counting!

Sartorius

Jan 21, 2020

IncuCyte has now reached over 2,000 cited publications! Search our publications filter to see what exciting research is...

Development of superparamagnetic nanoparticles as an efficient contrast agent for multimodal imaging

Bruker Biospin

Jan 16, 2020

Gadolinium‐based contrast agents are used to provide diagnostic information in clinical magnetic resonance...

IncuCyte live-cell analysis system for live-cell visualization of neuronal activity

Sartorius

Jan 13, 2020

A major impediment to studying diseases affecting the human nervous system is the ability to monitor, analyze, and...

Jan 26, 2020

Latest updates to Bionano Access and Bionano Solve

BioNano Genomics

Jan 8, 2020

Bionano Access v1.5 and Bionano Solve v3.5 are the latest updates to Bionano visualization software and analysis tools....

The qNano Gold: Obtain the most detailed Information for each individual nanoparticle

IZON

Jan 6, 2020

The qNano Gold measures particles using the Tunable Resistive Pulse Sensing (TRPS) principle. TRPS is the most powerful...

The importance of titrating Viability dyes on the Aurora Spectral Cytometer

Cytek Biosciences

Jan 2, 2020

What is the best way to decide when your viability is titrated properly? Unlike titrating antibodies how to determine...

Jan 26, 2020

New analysis open modules expand capabilities of Guava Muse flow cytometer

Luminex

Dec 30, 2019

Sophisticated cell analysis doesn’t have to be complicated or costly. With the Guava Muse cell analyzer, you can now...

Show all topics (10)

The effects of hypoxia and hydrostatic pressure in translational research

Apr 16, 2018

The characterization of the tumor microenvironment includes mechanotransduction, hypoxia, acidosis, and tissue remodeling. These factors can influence the gene and protein expression of variouse cell types that make up the tumor as well as influence the selection of cells that can thrive in a given microenvironment; however, typical cancer cell culture rarely uses hypoxia and pressure onr utilizes substrates similar to the native extracellular matrix. Cancer cell lines and PBMCs differentially express immunotherapeutic targets, at low oxygen and high pressure culturing conditions, resulting in reduced expression of key drug targets.

Analysis of mRNA-seq data revealed alterations in gene expression profiles of immunotherapeutic and drug-target pathways involving CTLA-4 and AR signaling. In contrast, it was obsorved increased CD47 and CD44 expression at low oxzgen and high presure culturing conditions in cancer cell lines and immune cells. Therefore these results support the presence of physiologically-relevant drug targets in tumor cells characterized by low oxygen and high interstitial fluid preassure.

Application
Product news

Related technologies: Physiological cell culturing

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey