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QC Plex - Multiplicom

For the qualitative assessment of genomic DNA derived from FFPE tissues

The QC plex assay enables the qualitative and quantitative assessment of human DNA. The assay is based on a multiplex PCR using a standard PCR protocol, followed by agarose gel-electrophoresis or a microfluidic-based method.

Assessing the quality and quantity of FFPE-derived DNA can be a challenge before conducting any downstream PCR based MPS applications. Several factors contribute to this suboptimal performance of FFPE-derived DNA: (1) formalin fixation can lead to DNA crosslinking and DNA degradation; (2) presence of PCR inhibiting factors and (3) low DNA concentration. Measuring FFPE-derived DNA using spectrophotometric and/or fluorimetric methods provides information on the amount of DNA present but not on DNA integrity for which an extra measurement is required. However, often the amount of FFPE-derived DNA available is limited to perform further consecutive quality measurements to ensure reliable and reproducible assay results.

To address the above issues, Multiplicom has developed a simple, standardized, qualitative, multiplex PCR assay that can be used to determine the global FFPE-derived DNA quality prior to performing MASTR assays. The multiplex PCR generates 7 PCR fragments of different lengths of which 6 (100, 200, 300, 400, 600 and 700 bp) directly assess the DNA integrity and 1 DNA fragment (150 bp) serves as a positive PCR control fragment to control for the absence of PCR inhibitors.  Data generated from the peak areas are used to calculate the DNA Quality Coefficient (DQC) value and DNA input requirement via the DQC Calculator.

Note: This assay is to be used for research use only.

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http://www.multiplicom.com/product/qc-plex
Petra Kupková

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Petra

Kupková

+420 731 426 051

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