Accelerate to discover

Back to filter

Related topics

Live animal imaging - Humane animal research

Bruker Biospin

Oct 26, 2020

Drawing on over 50 years of expertise in the life and animal sciences, Bruker designs preclinical imaging systems that...

Meet the Expert - Brent Barre interview

emka TECHNOLOGIES

Oct 21, 2020

Learn what a researcher at the forefront of COVID-19 research has to say about his current research environment.

Automated fever & hypothermia detection

emka TECHNOLOGIES

Oct 14, 2020

As one of the oldest clinical indicators of disease in mammals, temperature is a crucial parameter to monitor,...

Multi-modal molecular imaging driving pre-clinical research & pharmaceutical development

Bruker Biospin

Oct 6, 2020

Multi-modal molecular imaging at Massachusetts General Hospital – driving pre-clinical research and patient pathways...

Oct 26, 2020

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

The Role of Micro-CT in Imaging Breast Cancer Specimens

Bruker Biospin

Jun 17, 2020

The goal of breast cancer surgery is to remove all of the cancer with a minimum of normal tissue, but absence of full...

Bruker´s micro-CT used for some of the World’s First in vivo COVID-19 Imaging Experiments

Bruker Biospin

Jun 9, 2020

Bruker's BioSpin PreClinical Imaging Division reports the use of the Bruker SkyScan 1278 in vivo micro-CT system at the...

Oct 26, 2020

A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Spectral Instruments Imaging

Jun 3, 2020

Soft tissue sarcomas pose a significant clinical challenge, with approximately 12,000 new diagnoses and an estimated...

Show all topics (10)

ACEA NovoCyte used in cell membrane enrichment research

Apr 4, 2016

Efficient cellular uptake of nanoparticles is crucial for modulating the cell behaviors as well as dictating the cell fate. In this work, by using two commercial reagents (the membrane modification reagent “cholesterol–PEG–biotin” and the avidin-modified quantum dots (QDs) “QD–avidin”), we achieved the enhanced plasma membrane enrichment and endocytosis of fluorescent QDs in cancer cells through cell surface engineering. The QD–cell interaction involved two stages: adsorption and internalization. After incubation with cholesterol–PEG2k–biotin, the cell membrane was engineered with biotin groups that would actively recruit QD–avidin to the cell surface within 1 min. This fast adsorption process could realize high quality and photostable plasma membrane imaging, which is simple, low-cost and generally applicable as compared with the previously reported membrane protein/receptor labeling-based QD imaging. After that, the QDs attached on the cell surface underwent the internalization process and 12 h later, almost all the QDs were internalized through endocytosis. Notably, we found that the internalization of QDs was not via common endocytosis pathways (such as clathrin- or caveolae-mediated endocytosis or macropinocytosis) but more likely via lipid raft-dependent endocytosis. In contrast, without cell surface engineering, the QD–avidin showed negligible cellular uptake. The results demonstrate that cell surface engineering is an efficient strategy to image the plasma membrane and increase cellular uptake of nanoparticles, and will be potentially applied to enhance the efficacy of nanomedicines when therapeutic nanoparticles are used.

Read more

Scientific paper

Related technologies: Conventional flow cytometry

Brand profile

Agilent technologies

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey