Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Automated microscopy and image analysis
Real-time, label free cell analysis
Nano and micro particle analysis

Accelerate to discover
Related topics
Outstanding post-sort sample recovery on the WOLF Cell Sorter
Jun 14, 2022
The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...
Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters
Jun 3, 2022
Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing
Jun 1, 2022
Recent article from February 2022 shoes how size selection improved viral read mapping by over 90% for begomoviruses.
Extracellular Vesicle Purity Enhanced by Gen 2 qEV Columns With Customised Proprietary Resin
May 4, 2022
Izon Science has announced the launch of Gen 2, the enhanced range of qEV columns for size-exclusion...
Multi-modal PET drives interdisciplinary preclinical imaging
Feb 23, 2022
Preclinical imaging (PCI) plays a vital role in understanding the biological processes behind disease states at the...
Quantitative 3D Optical Imaging for Lago
Feb 21, 2022
Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...
Reduces fMRI-BOLD Response in the Brain
Feb 18, 2022
fMRI studies often involve the electrical stimulation of i.e. the forepaw, detecting where this stimulation is...
Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
Nov 23, 2021
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...
Apr 4, 2016
Efficient cellular uptake of nanoparticles is crucial for modulating the cell behaviors as well as dictating the cell fate. In this work, by using two commercial reagents (the membrane modification reagent “cholesterol–PEG–biotin” and the avidin-modified quantum dots (QDs) “QD–avidin”), we achieved the enhanced plasma membrane enrichment and endocytosis of fluorescent QDs in cancer cells through cell surface engineering. The QD–cell interaction involved two stages: adsorption and internalization. After incubation with cholesterol–PEG2k–biotin, the cell membrane was engineered with biotin groups that would actively recruit QD–avidin to the cell surface within 1 min. This fast adsorption process could realize high quality and photostable plasma membrane imaging, which is simple, low-cost and generally applicable as compared with the previously reported membrane protein/receptor labeling-based QD imaging. After that, the QDs attached on the cell surface underwent the internalization process and 12 h later, almost all the QDs were internalized through endocytosis. Notably, we found that the internalization of QDs was not via common endocytosis pathways (such as clathrin- or caveolae-mediated endocytosis or macropinocytosis) but more likely via lipid raft-dependent endocytosis. In contrast, without cell surface engineering, the QD–avidin showed negligible cellular uptake. The results demonstrate that cell surface engineering is an efficient strategy to image the plasma membrane and increase cellular uptake of nanoparticles, and will be potentially applied to enhance the efficacy of nanomedicines when therapeutic nanoparticles are used.
Related technologies: Conventional flow cytometry
Brand profile
Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
More info at:
www.aceabio.com