Accelerate to discover

Back to filter

Related topics

Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

Sage Science

Mar 24, 2021

Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

Preclinical lung imaging offers effective evaluation of drug candidates

Bruker Biospin

Mar 1, 2021

The incidence of respiratory disease is increasing throughout the world, and the demand for safe, effective drugs to...

Towards silent and distortion-free fMRI with Zero Echo Time MRI

Bruker Biospin

Feb 9, 2021

By using fast imaging techniques capable of scanning large brain regions within short durations, continuous snapshots...

A Glimpse on physiological monitoring for the in vivo SKYSCAN Systems

Bruker Biospin

Jan 14, 2021

This past year has proven once again that in vivo imaging is an important aspect in the microCT world. For longitudinal...

Apr 20, 2021

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 28, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Don’t Let Waste Anesthetic Gases Harm You!

RWD

Dec 21, 2020

Many projects have been devoted to the study of the correlation between anesthetics and Alzheimer’s disease....

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

Dec 10, 2020

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Apr 20, 2021

Do you have our CellRad benchtop irradiator? It’s the only one on the market!

Precision X-Ray

Dec 8, 2020

The CellRad is a smaller, simpler, safer, and more cost effective alternative to radioisotope or high-powered X-ray...

Show all topics (10)

ACEA NovoCyte used in cell membrane enrichment research

Apr 4, 2016

Efficient cellular uptake of nanoparticles is crucial for modulating the cell behaviors as well as dictating the cell fate. In this work, by using two commercial reagents (the membrane modification reagent “cholesterol–PEG–biotin” and the avidin-modified quantum dots (QDs) “QD–avidin”), we achieved the enhanced plasma membrane enrichment and endocytosis of fluorescent QDs in cancer cells through cell surface engineering. The QD–cell interaction involved two stages: adsorption and internalization. After incubation with cholesterol–PEG2k–biotin, the cell membrane was engineered with biotin groups that would actively recruit QD–avidin to the cell surface within 1 min. This fast adsorption process could realize high quality and photostable plasma membrane imaging, which is simple, low-cost and generally applicable as compared with the previously reported membrane protein/receptor labeling-based QD imaging. After that, the QDs attached on the cell surface underwent the internalization process and 12 h later, almost all the QDs were internalized through endocytosis. Notably, we found that the internalization of QDs was not via common endocytosis pathways (such as clathrin- or caveolae-mediated endocytosis or macropinocytosis) but more likely via lipid raft-dependent endocytosis. In contrast, without cell surface engineering, the QD–avidin showed negligible cellular uptake. The results demonstrate that cell surface engineering is an efficient strategy to image the plasma membrane and increase cellular uptake of nanoparticles, and will be potentially applied to enhance the efficacy of nanomedicines when therapeutic nanoparticles are used.

Read more

Scientific paper

Related technologies: Conventional flow cytometry

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey