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Small animal 13C-hyperpolarized MRI

Bruker Biospin

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13C-Hyperpolarized molecules provide a unique signature for molecular processes relevant to a range of disease...

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer xenografts

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Pancreatic ductal adenocarcinoma has been increasing by 0.5% per year in the United States. This study describes the...

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Spectral Instruments Imaging

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In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. The...

Improved differentiation of Retinal Organoids stem cells derived using rotating-wall vessels

Synthecon

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Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a...

Feb 20, 2019

CT‐analyzer plug‐ins: ROI shrink‐wrap and primitive ROI

Bruker Biospin

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The ROI shrink‐wrap gives the possibility to automatically generate a region‐of‐interest by adapting to the shape of...

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Fluxion Biosciences

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Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

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MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

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Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies

Etaluma

Dec 6, 2018

Accurate labeling of endogenous proteins for advanced light microscopy in living cells remains challenging. Nanobodies...

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Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation

Aug 23, 2016

Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitochondria during differentiation has yet to be fully elucidated. Mitochondria mediate a cells response to altered energy requirements (e.g. cardiomyocyte contraction) and, as such, the mitochondrial phenotype is likely to change during the dynamic process of hESC differentiation. We demonstrate that manipulating mitochondrial biogenesis alters mesendoderm commitment. To investigate mitochondrial localisation during early lineage specification of hESCs we developed a mitochondrial reporter line, KMEL2, in which sequences encoding the green fluorescent protein (GFP) are targeted to the mitochondria. Differentiation of KMEL2 lines into the three germ layers showed that the mitochondria in these differentiated progeny are GFP positive. Therefore, KMEL2 hESCs facilitate the study of mitochondria in a range of cell types and, importantly, permit real-time analysis of mitochondria via the GFP tag. Live cell images of LDS-751 stained hESC were taken using an Amnis ImageStream cytometer.

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