Accelerate to discover

Back to filter

Related topics

Download today your IsoPlexis e-book: understanding immune Cell Function In Cancer

IsoPlexis

Dec 1, 2021

Understanding complex immune-cancer interactions is critical to identifying therapeutic targets, devising novel avenues...

IsoPlexis technology: accelerating decisions in immunotherapy with predictive single-cell response

IsoPlexis

Nov 25, 2021

The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell...

Introducing isoLight system - Automated Cellular Proteomics hub

IsoPlexis

Nov 4, 2021

The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell...

Introducing Cytek NEW 25-Color Immunoprofiling Assay & Kit!

Cytek Biosciences

Oct 25, 2021

To unleash the power of the Cytek full spectrum cytometry system, Cytek scientists designed and developed the 25-Color...

Jan 26, 2022

Curiox webinar: Laminar Wash enables single-cell multiomics and cell hashing

Curiox

Oct 11, 2021

Professor Mats Bemark’s lab at the University of Gothenburg in Sweden studies how the immune system responds to...

IncuCyte Cell-by-Cell : advancing label-free morphological analysis

Sartorius & Essen BioScience

Sep 29, 2021

Cell morphology reveals valuable insight into cell health and status, and considerable heterogeneity exists in even the...

Deep-Learning, Label-Free, Quantitative Cell Segmentation with IncuCyte

Sartorius & Essen BioScience

Sep 24, 2021

Sartorius Unveils LIVECell, a Deep-Learning Dataset for Label-Free, Quantitative Cell Segmentation in Nature Methods.

Jan 26, 2022

See What's Possible with the PippinHT

Sage Science

Sep 14, 2021

Programmable DNA size selection enables new discoveries by helping optimize the library detected by a sequencer. That’s...

Show all topics (10)

Cancer Immunotherapy Applications of xCELLigence Real-Time Cell Analysis (RTCA) Systems

Mar 3, 2016

Many in vitro assays have been developed to screen and evaluate the efficacy of immune cell-mediated killing. The most common of these is the release assay where effector cell-mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. Endogenous biomolecules (such as lactate dehydrogenase) or previously added exogenous labels (such as the radioisotopes 51Cr or 111In) that leak into the media are then measured as an indirect readout of the damage caused by effector cells.

Alternative endpoint methods include flow cytometry, ELISA-based granzyme measurement, and morphometric analyses by microscopy. Although traditional end-point assays still serve as important tools for evaluating the efficacy of immune cell-mediated killing, there is a critical need for more efficient, homogeneous, automated assays (requiring less hands-on time) that aren’t hampered by the artifacts and hassle of using labels and that can provide quantitative results.

ACEA’s xCELLigence Real-Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor cell status including cell number, shape/size and attachment. The major distinguishing features of this technology include enhanced sensitivity, the preclusion of labels and, importantly, kinetic measurement of cell health/behavior.

Read the application note.

Product news

Related technologies: Real-time, label free cell analysis

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

Located inside a tissue culture incubator, the RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey