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High-frequency Ultrasound System For Preclinical Imaging

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The Prospect T1 is an innovative high-frequency ultrasound system designed specifically for in vivo preclinical imaging...

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It is important for the analysis of data from space experiments to distinguish unspecific stress reactions from...

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Mounting evidence implicated the classical complement pathway (CP) in normal brain development, and the pathogenesis of...

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Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging...

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Co-localization of an antibody-drug conjugate to endosomes or lysosomes

Jun 20, 2016

Using imaging flow cytometry has been possible to optimize an innovative method for studying co-localization of probes by combining the automatic and rapid collection of images with the objective quantification of probe location and intensity.The correlation analysis built in to the IDEAS image analysis software provides robust statistically significant results on large numbers of cells so that phenotypically distinct subsets of cells can be analyzed. In this experiment we use the capabilities of the ImageStreamX system, to measure the transit of a fluorescently labeled antibody-drug conjugate (ADC) through the cellular endocytic pathway. The high spatial resolution afforded by the ImageStreamX system allowed accurate measurement of ADC co-localization to endosomes and lysosomes.

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Scientific paper

Related technologies: Imaging flow cytometry

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Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

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