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Small animal 13C-hyperpolarized MRI

Bruker Biospin

Feb 8, 2019

13C-Hyperpolarized molecules provide a unique signature for molecular processes relevant to a range of disease...

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer xenografts

Spectral Instruments Imaging

Jan 30, 2019

Pancreatic ductal adenocarcinoma has been increasing by 0.5% per year in the United States. This study describes the...

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Spectral Instruments Imaging

Jan 17, 2019

In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. The...

Improved differentiation of Retinal Organoids stem cells derived using rotating-wall vessels

Synthecon

Jan 15, 2019

Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a...

Feb 20, 2019

CT‐analyzer plug‐ins: ROI shrink‐wrap and primitive ROI

Bruker Biospin

Jan 7, 2019

The ROI shrink‐wrap gives the possibility to automatically generate a region‐of‐interest by adapting to the shape of...

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Fluxion Biosciences

Dec 19, 2018

Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

Dec 17, 2018

MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

Feb 20, 2019

Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies

Etaluma

Dec 6, 2018

Accurate labeling of endogenous proteins for advanced light microscopy in living cells remains challenging. Nanobodies...

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Co-localization of an antibody-drug conjugate to endosomes or lysosomes

Jun 20, 2016

Using imaging flow cytometry has been possible to optimize an innovative method for studying co-localization of probes by combining the automatic and rapid collection of images with the objective quantification of probe location and intensity.The correlation analysis built in to the IDEAS image analysis software provides robust statistically significant results on large numbers of cells so that phenotypically distinct subsets of cells can be analyzed. In this experiment we use the capabilities of the ImageStreamX system, to measure the transit of a fluorescently labeled antibody-drug conjugate (ADC) through the cellular endocytic pathway. The high spatial resolution afforded by the ImageStreamX system allowed accurate measurement of ADC co-localization to endosomes and lysosomes.

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