Accelerate to discover

Back to filter

Related topics

PET/CT Si78: Versatility in microCT dose, speed, & resolution

Bruker Biospin

May 9, 2019

The Bruker PET/CT Si78 now pairs total-body PET and SkyScan microCT technology. This combination offers the advanced...

Cell by cell lung cancer marker profiling using high-parameter spectral flow cytometry

Cytek Biosciences

May 2, 2019

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, accounting for 80-85% cases of lung cancer....

In Vivo Bioluminescence Imaging of Cobalt Accumulation in a Mouse Model

Spectral Instruments Imaging

Apr 26, 2019

As a trace element nutrient, cobalt is critical for both prokaryotes and eukaryotes. In the current study, a turn-on...

Synthesis and characterization of functional nanofilm-coated live Immune cells using CQ1

Yokogawa

Apr 10, 2019

Layer-by-layer assembly techniques have been extensively studied in cell biology because of their simplicity of...

May 23, 2019

Novel imaging-based assay for hERG channel inhibition using iPS-derived Cardiomyocytes

Yokogawa

Apr 4, 2019

A large percentage of drugs fail in clinical studies due to cardiac toxicity, such as drug-induced QT...

Modified Beta-carotene-bound albumin nanoparticles for breast tumor ablation

Spectral Instruments Imaging

Mar 29, 2019

The albumin nanoparticles were fabricated using a beta-carotene cross-linker via the Nab technique. These new albumin...

Spectral flow cytometric analysis of loss-of-function mutations in granular cell tumors

Cytek Biosciences

Mar 29, 2019

Granular cell tumors (GCTs) are rare tumors that can arise in multiple anatomical locations,and are characterized by...

May 23, 2019

Cell plasticity associated to circulating tumor cells from prostate cancer patients

Fluxion Biosciences

Mar 13, 2019

Treatment resistance is associated with tumor cells molecular plasticity. In metastatic castration resistant prostate...

Show all topics (10)

Co-localization of an antibody-drug conjugate to endosomes or lysosomes

Jun 20, 2016

Using imaging flow cytometry has been possible to optimize an innovative method for studying co-localization of probes by combining the automatic and rapid collection of images with the objective quantification of probe location and intensity.The correlation analysis built in to the IDEAS image analysis software provides robust statistically significant results on large numbers of cells so that phenotypically distinct subsets of cells can be analyzed. In this experiment we use the capabilities of the ImageStreamX system, to measure the transit of a fluorescently labeled antibody-drug conjugate (ADC) through the cellular endocytic pathway. The high spatial resolution afforded by the ImageStreamX system allowed accurate measurement of ADC co-localization to endosomes and lysosomes.

Read more

Scientific paper

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Amnis

Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey