Accelerate to discover

Back to filter

Related topics

Hypoxia further exacerbates myopathy in broilers via alteration of satellite cell fate

COY laboratory products

Aug 2, 2021

Woody breast (WB) condition has created a variety of challenges for the global poultry industry. To date, there are no...

High quality Bioluminescence and X-Ray analysis in lung squamous cell carcinoma

Spectral Instruments Imaging

Jul 21, 2021

Spectral Instruments Imaging provides unrivaled sensitivity for bioluminescence, fluorescence and X-Ray for in vivo...

Webinar recording: Autofluorescence management - the power of Spectral Flow Cytometry

Cytek Biosciences

Jul 15, 2021

One of the key advantages of spectral flow cytometry is its ability to extract sample autofluorescence, making it much...

Top 10 Pharma companies have chosen CYTEK for their flow cytometry facility

Cytek Biosciences

Jul 13, 2021

6 of Top 10 Pharma Companies (Based on total group revenues) have chosen CYTEK - full spectrum flow cytometer for their...

Aug 3, 2021

Introducing IZON Exoid: accurate analysis of nanoparticles from 1 to 1000 nm

IZON

Jul 7, 2021

The IZON Exoid is the only standardisable method of measuring the entire nano range (1–1000 nm).

Webinar recording: Super-Resolution imaging of Chromatin organization in health and disease

ONI

Jul 6, 2021

Understanding how cells organize the array of components within their membranous confines can not only provide insight...

Aug 3, 2021

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jun 23, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma. BEZ235 is an effective dual...

Show all topics (10)

Confocal laser endomicroscopy in head and neck malignancies

Mar 8, 2017

Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients outcome. The feasibility of the tumor antigens epidermal growth factor receptor and epithelial cell adhesion molecule for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas, and represent prognostic and therapeutic tumor target molecules. FITC-conjugated EGF-R- and EpCAM-specific antibodies served as molecular tools for the detection of antigen-positive cells using the CLE technology. Specificity of both antibodies and their ability to discriminate tumor from non-tumor cells were assessed in vitro with human fibroblasts and PCI-1 HNSCC cell lines, and ex vivo on primary HNSCC samples and healthy mucosa. Antigen specificity of the used EpCAM-specific antibody was superior to that of the EGF-R-specific antibody both in vitro and ex vivo, and allowed visualization of cellular structures in CLE measurements. These results hold promise for possible future applications in humans.

Read more

Scientific paper
Application

Related technologies: Probe based confocal microscopy

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 255 700 886

Send Message

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey