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Development of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis

Oct 19, 2017

Group A streptococci (GAS) are the most common bacterial cause of acute pharyngitis and account for 15–30 % of cases of acute pharyngitis in children and 5–10 % of cases in adults. The real-time quantitative PCR (qPCR) based GAS detection assay in pharyngeal swab specimens was developed. The qPCR was carried out using four different Real-Time PCR system: Biorad CFX Connect (Bio-Rad Laboratories, USA), LightCycler 480 (Roche Applied Science, USA), StepOne Plus (Life Technologies, USA) and Xxpress (BJS Biotechnologies, UK). Duration of the total assay for 24 samples including preanalytical processing and analysis was 50±2, 51±3, 55±3 and 42±3 min for Biorad CFX Connect, LightCycler 480, StepOne Plus and Xxpress respectively.

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