Accelerate to discover

Back to filter

Related topics

Advancing Alzheimer's disease rsearch with IncuCyte S3 for Neuroscience

Sartorius

Nov 12, 2019

IncuCyte S3 Live-Cell Analysis System for Neuroscience: enables real-time automated measurements of the dynamic changes...

Webinar recording: Analysis of extracellular vesicles by AMNIS imaging flow cytometer

Luminex

Nov 4, 2019

Although conventional flow cytometry is limited to measure small particles down to approximately 300 nm – 500 nm, an...

Targeted mitochondrial DNA extraction and enrichment using the SageHLS

Sage Science

Oct 31, 2019

Sage Science introduced a novel approach for enriching mitochondrial DNA for sequence analysis without PCR or gradient...

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer at a single low dose

Spectral Instruments Imaging

Oct 24, 2019

Pancreatic ductal adenocarcinoma (PDAC) has been increasing by 0.5% per year in the United States. This study describes...

Nov 22, 2019

New code dedicated to esophageal procedures with Confocal Laser Endomicroscopy

Mauna Kea Technologies

Oct 21, 2019

Announcement of the creation of a new specific procedural code to be added to the Common Classification of Medical...

VisualSonics AutoLV

VisualSonics FujiFilm

Sep 27, 2019

VisualSonics AutoLV allow functional and anatomical analysis of the left ventricle in one click using the power of...

New study resolves helicase dynamics and interactions during DNA replication using C-Trap

LUMICKS

Aug 30, 2019

Researchers from Rockefeller University used a C-Trap optical tweezers – fluorescence microscopy system to observe the...

Nov 22, 2019

Low-dose maging in a new preclinical total-body PET/CT scanner

Bruker Biospin

Aug 28, 2019

Ionizing radiation constitutes a health risk to imaging scientists and study animals. Both PET and CT produce ionizing...

Show all topics (10)

Evaluation of signaling and drug response of cancer cells culture in 2D vs 3D

Jan 25, 2017

Spheroids showed relative lower activities in the AKT, mammalian target of rapamycin (mTOR) and S6K (also known as RPS6KB1) signaling pathway compared to cells cultured in two dimensions. We identified spatial alterations in signaling, as the level of phosphorylated RPS6 decreased from the spheroid surface towards the center, which closely coordinated with the tumor areas around vessels in vivo. These 3D models displayed augmented antitumor responses to AKT–mTOR–S6K or mitogen-activated protein kinase (MAPK) pathway inhibition compared to those in 2D models. Inhibition of AKT–mTOR–S6K resulted in elevated ERK phosphorylation in 2D culture, whereas under these conditions, ERK signaling was reduced in spheroids. Inhibition of MEK1 (also known as MAP2K1) led to decreased AKT–mTOR–S6K signaling in 3D but not in 2D culture. These data indicate a distinct rewiring of signaling in 3D culture and during treatment. Detached tumor-cell clusters in vessels, in addition to circulating single tumor cells, play a putative role in metastasis in human cancers. Hence, the understanding of signaling in spheroids and the responses in the 3D models upon drug treatment might be beneficial for anti-cancer therapies.

Read more

Scientific paper

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

TissueGnostics

TissueGnostics develops systems for automated identification and functional characterization of single cells in tissue sections, cultured and smeared cells as well as TMA. This includes automated image acquisition and image processing for immunohistochemistry, multi-chanel immunofluorescence and hematology.

Related products

Tissue analysis system for immunohistochemistry and immunofluorescence. TissueFAXSi Plus draws together features of HistoFAXS and TissueFAXS systems.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey