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F.I.S.H. analysis using ImageStream, multispectral imaging flow cytometry

Aug 12, 2016

Amnis Corporation has developed the ImageStream multispectral imaging flow cytometer. The platform combines the morphological capabilities of multiple forms of microscopy with the sample handling and quantitative power of flow cytometry. The high throughput capabilities of the ImageStream instrument are well suited for chromosomal Fluorescent In Situ Hybridization (FISH) analysis of large numbers of cells. Amnis’ IDEAS software package allows quantitative, objective spot counting and analysis. In order to convert from a slide format to a flow format Amnis has developed FISH In Suspension (FISH-IS) protocols. Applications include the detection of aneuploidy in sperm and other cell types, detection of gene amplification and translocation associated with cancer, and detection of residual disease after bone marrow transplantation. Presented is the high throughput quantitation of aneuploidy in human sperm using probes for chromosomes 8 and Y. 

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Related technologies: Imaging flow cytometry

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Riccardo Pasculli

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Amnis

Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

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