Accelerate to discover

Back to filter

Related topics

High quality Bioluminescence and X-Ray analysis in lung squamous cell carcinoma

Spectral Instruments Imaging

Jul 21, 2021

Spectral Instruments Imaging provides unrivaled sensitivity for bioluminescence, fluorescence and X-Ray for in vivo...

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jun 23, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma. BEZ235 is an effective dual...

Quantitative two-photon microscopy imaging analysis of human skin

IVIM Technology

Jun 21, 2021

Transdermal skin delivery is a method to transport various topical formulations to a deeper skin layer non-invasively....

Elevated NSD3 histone methylation activity drives squamous cell lung cancer

Spectral Instruments Imaging

Jun 8, 2021

This work identifies NSD3 as a principal 8p11-12 amplicon-associated oncogenic driver in LUSC, and suggests that...

Aug 1, 2021

Diffusion functional MRI reveals global brain network functional abnormalities

Bruker Biospin

May 13, 2021

Repetitive behaviors are one of the behavioral hallmarks of neuropsychiatric disorders.
Resting state functional
...

Cytek Spectral cytometers : taking science one step further with 200+ Publications

Cytek Biosciences

Apr 26, 2021

Cytek Aurora is helping researchers around the world take their science further. See how in the 200+ publications in...

Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

Sage Science

Mar 24, 2021

Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

Aug 1, 2021

Preclinical lung imaging offers effective evaluation of drug candidates

Bruker Biospin

Mar 1, 2021

The incidence of respiratory disease is increasing throughout the world, and the demand for safe, effective drugs to...

Show all topics (10)

Foot-and-mouth deseases virus research using IncuCyte ZOOM

Apr 21, 2016

Probing the molecular interactions within the foot-and-mouth disease virus (FMDV) RNA replication complex has been restricted in part by the lack of suitable reagents. Random insertional mutagenesis has proven an excellent method to reveal domains of proteins essential for virus replication as well as locations that can tolerate small genetic insertions. Such insertion sites can subsequently be adapted by the incorporation of commonly used epitope tags, facilitating their detection with commercially available reagents. In this study, we used random transposon-mediated mutagenesis to produce a library of 15 nt insertions in the FMDV nonstructural polyprotein. Using a replicon-based assay, we isolated multiple replication-competent as well as replication-defective insertions. We adapted the replication-competent insertion sites for the successful incorporation of epitope tags within FMDV non-structural proteins for use in a variety of downstream assays. Additionally, we showed that replication of some of the replication-defective insertion mutants could be rescued by co-transfection of a ‘helper’ replicon, demonstrating a novel use of random mutagenesis to identify intergenomic transcomplementation. Both the epitope tags and replication-defective insertions identified here will be valuable tools for probing interactions within picornavirus replication complexes.

Read more

Scientific paper

Related technologies: Real-time, label free cell analysis

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey