Accelerate to discover

Back to filter

Related topics

IncuCyte Live-Cell Analysis System - nearly 3,000 publications and counting!

Sartorius

Jan 21, 2020

IncuCyte has now reached over 2,000 cited publications! Search our publications filter to see what exciting research is...

Development of superparamagnetic nanoparticles as an efficient contrast agent for multimodal imaging

Bruker Biospin

Jan 16, 2020

Gadolinium‐based contrast agents are used to provide diagnostic information in clinical magnetic resonance...

IncuCyte live-cell analysis system for live-cell visualization of neuronal activity

Sartorius

Jan 13, 2020

A major impediment to studying diseases affecting the human nervous system is the ability to monitor, analyze, and...

High molecular weight DNA from bone marrow aspirates samples in 4 hrs

BioNano Genomics

Dec 23, 2019

With Bionano Prep SP BMA DNA Isolation kit, transform the way you are isolating UHMW DNA from this complex sample type...

Jan 22, 2020

Cutting-edge analysis of Extracellular Microparticles using ImageStream Imaging Flow Cytometry

Amnis

Dec 16, 2019

Interest in extracellular vesicle biology has exploded in the past decade, since these microstructures seem endowed...

Bruker MRI consistent quantification

Bruker Biospin

Nov 29, 2019

Regardless of the method, stability and reproducibility are a must for many laboratories. Identical protocols must...

Targeted sequencing of highly homologous genes using Cas9 digestion

Sage Science

Nov 25, 2019

Integrated DNA extraction, digestion, and size-selection using HLS-CATCH were introduced for addressing the problem of...

Jan 22, 2020

Advancing Alzheimer's disease research with IncuCyte S3 for Neuroscience

Sartorius

Nov 12, 2019

IncuCyte S3 Live-Cell Analysis System for Neuroscience: enables real-time automated measurements of the dynamic changes...

Show all topics (10)

Foot-and-mouth deseases virus research using IncuCyte ZOOM

Apr 21, 2016

Probing the molecular interactions within the foot-and-mouth disease virus (FMDV) RNA replication complex has been restricted in part by the lack of suitable reagents. Random insertional mutagenesis has proven an excellent method to reveal domains of proteins essential for virus replication as well as locations that can tolerate small genetic insertions. Such insertion sites can subsequently be adapted by the incorporation of commonly used epitope tags, facilitating their detection with commercially available reagents. In this study, we used random transposon-mediated mutagenesis to produce a library of 15 nt insertions in the FMDV nonstructural polyprotein. Using a replicon-based assay, we isolated multiple replication-competent as well as replication-defective insertions. We adapted the replication-competent insertion sites for the successful incorporation of epitope tags within FMDV non-structural proteins for use in a variety of downstream assays. Additionally, we showed that replication of some of the replication-defective insertion mutants could be rescued by co-transfection of a ‘helper’ replicon, demonstrating a novel use of random mutagenesis to identify intergenomic transcomplementation. Both the epitope tags and replication-defective insertions identified here will be valuable tools for probing interactions within picornavirus replication complexes.

Read more

Scientific paper

Related technologies: Real-time, label free cell analysis

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey