Accelerate to discover

Back to filter

Related topics

IncuCyte webinar: Latest developments in live-cell analysis & advanced cell models

Sartorius

Sep 26, 2018

Biomedical researchers are increasingly using advanced human cell models for translational biology and therapeutic...

IncuCyte webinar: Live-cell analysis of cell subsets and heterogeneity

Sartorius

Sep 3, 2018

Discover the new IncuCyte S3 cell-by-cell analysis software module. The innovative module allow label free analysis to...

Webinar Tuning Microenvironment to Control Cells for Improved Disease Modeling & Immunotherapy Work

Xcell Biosciences

Jul 16, 2018

The company producing Avatar, xCELL biosciences have organized a webinar about the tuning of microenvironment to...

How microCT can provide insight into dynamic disease processes webinar

Bruker Biospin

Jun 19, 2018

Professor Vande Velde will describe how high-field MRI and low dose microCT complement each other; where microCT gives...

Oct 15, 2018

xCELLIgence webinar: label-free real-time cell analysis technology for Immuno-Oncology

Acea Biosciences

Jun 5, 2018

Cancer immunotherapies harness and enhance the innate power of the immune system to eliminate cancer cells and have...

Photoacoustic Imaging of placental oxygenation

VisualSonics FujiFilm

May 16, 2018

Watch the new webinar about photoacoustick imaging presented by Dr. Liliya M. Yamaleyeva,  Assistant Professor in the...

BioFlux webinar: Introducing new plates and capabilities of BioFlux microfluidic system

Fluxion Biosciences

May 13, 2018

BioFlux biofilm assays can now be performed on the popular catheter material polydimethylsiloxane (PDMS).  Silicone...

Oct 15, 2018

IncuCyte webinar: advanced 3D cell models providing greater biological insight

Sartorius

Apr 30, 2018

Cancer researchers are increasingly turning to more complex live-cell assay systems that recapitulate key aspects of...

Show all topics (10)

Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity

Aug 21, 2017

September 7, 2017, 7 pm CEST

Chia-Wei Li, PhD, The University of Texas M. D. Anderson Cancer Center 

Here we reveal PD-L1’s immunosuppression activity is stringently modulated by the spatial restraints between ubiquitination and N-linked glycosylation. We identified GSK3beta as a novel PD-L1 interacting protein capable of inducing phosphorylation dependent proteasome degradation. The essence of PD-L1 N192, N200 and N219 glycosylation suggests it antagonizes GSK3beta binding. In this regard, only non-glycosylated PD-L1 forms a complex with GSK3beta and beta-TrCP. More importantly, activation of GSK3beta-mediated PD-L1 degradation by TKI can reduce membrane PD-L1 expression and thereby enhance anti-tumor immunity. To test T cell-mediated cancer cell killing in vitro, we employed a time lapse microscopy, IncuCyte, to monitor RFP-tagged BT549 cells and activated PBMC interaction over a five day period. Massive cancer cell death was observed in glycosylation deficient cancer cells, suggesting glycosylation of PD-L1 is required for its immunosuppression. Together, our results link ubiquitination and glycosylation pathways with stringent regulation of PD-L1, proposing a new therapeutic strategy to enhance cancer immune therapy efficacy.

Learning objectives:

  • Molecular mechanism of PD-L1 regulation in cancer cells
  • Immunosuppressive nature of triple negative breast cancer
  • Using in vitro co-culture system to analyze T cell-mediated cancer cell killing effect

Register now

Webinar

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

Live Cell Imaging in Your Incubator

show detail

Real-time automated measurements of cell health, movement and function, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey