Accelerate to discover

Back to filter

Related topics

Cytek Spectral cytometers : taking science one step further with 200+ Publications

Cytek Biosciences

Apr 26, 2021

Cytek Aurora is helping researchers around the world take their science further. See how in the 200+ publications in...

Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

Sage Science

Mar 24, 2021

Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

Preclinical lung imaging offers effective evaluation of drug candidates

Bruker Biospin

Mar 1, 2021

The incidence of respiratory disease is increasing throughout the world, and the demand for safe, effective drugs to...

Towards silent and distortion-free fMRI with Zero Echo Time MRI

Bruker Biospin

Feb 9, 2021

By using fast imaging techniques capable of scanning large brain regions within short durations, continuous snapshots...

May 12, 2021

A Glimpse on physiological monitoring for the in vivo SKYSCAN Systems

Bruker Biospin

Jan 14, 2021

This past year has proven once again that in vivo imaging is an important aspect in the microCT world. For longitudinal...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 28, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Don’t Let Waste Anesthetic Gases Harm You!

RWD

Dec 21, 2020

Many projects have been devoted to the study of the correlation between anesthetics and Alzheimer’s disease....

May 12, 2021

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

Dec 10, 2020

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Show all topics (10)

Growth dynamics of fetal human neural stem cells

Jun 23, 2017

This laboratory has developed methods for culture of fetal telencephalon-derived hNSC in multilayers by seeding on tissue culture-treated but otherwise unmodified plastic sur-faces at relatively high densities. Multilayers are inter-mediate between monolayers and neurospheres-cells interact with each other in local aggregations or clusters but also with the substrate via lamellipodia extending toward adjacent cell clusters. Proliferation expands the cluster area horizontally along the substrate as well as vertically in each expanding cluster. Migration of cells between clusters along the lamellipodia and subsequent proliferation is hypothe-sized to eventually unify previously discrete clusters. This culture mode produces large numbers of hNSC compared to both neurospheres and monolayers with the resulting cells suitable for differentiation control and transplantation. As culture of hNSC in multilayers is much less studied than monolayers and neurospheres, the present work explores quantitative parameters for successful propagation and main-tenance of hNSC in this culture modality. Understanding these parameters will further acceptance of this technique for the realization of these advantages.

Cells were transilluminated in brightfield at an oblique angle through the plastic dish cover with a white LED on a flexible cable. After plating, the dish was placed on the stage of the microscope inside a 37 °C incubator, a field of view was brought into focus, and successive images were acquired and stored as bitmaps at time intervals of 5 or 10 min for a collec-tion period of up to 14 days with custom software (LumaView). Image files were assembled into movies with Adobe Premier 6.5. In brief, each 4 MB bitmap image (1,280 × 800 pixels) in a time-lapse series was replicated into five successive video frames (760 × 480 pixels). The result-ing sequence of video frames was compressed by an MPEG-1 codec into a movie for playback at 30 frames per second. Total time-lapse collection periods and movie durations are noted in each movie legend.

Read more

 

Scientific paper

Related technologies: Live cell imaging

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Etaluma

With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.

Related products

The true color inverted brightfield microscopy with all of the LumaView software features of the fluorescent LumaScope

show detail

The original single color 488nm excitation fluorescence inverted microscopy

show detail

Lumascope 620 offers a larger sensor and 3-color fluorescence for the most powerful microscope offered anywhere at this price

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey