Accelerate to discover

Homepage>Discover>Hypoxia activates constitutive Luciferase reporter constructs

Dec 28, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Don’t Let Waste Anesthetic Gases Harm You!

Dec 21, 2020

Many projects have been devoted to the study of the correlation between anesthetics and Alzheimer’s disease....

Advances in Leukemia research using shear flow and Bioflux system

Dec 10, 2020

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Do you have our CellRad benchtop irradiator? It’s the only one on the market!

Dec 8, 2020

The CellRad is a smaller, simpler, safer, and more cost effective alternative to radioisotope or high-powered X-ray...

Dec 30, 2020

Dosimetric characterization of an X-ray irradiator for use with cells

Dec 4, 2020

This study aims to characterize an X-ray irradiator for use with cells using its internal parallel-plate ionization...

Modeling multiple myeloma-Bone Marrow interactions using Synthecon rotating-wall vessels

Dec 2, 2020

Multiple myeloma develops primarily inside the bone marrow microenvironment, that confers pro-survival signals and drug...

Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2

Nov 19, 2020

The development of a vaccine against COVID-19 is a hot topic for many research laboratories all over the world. Our aim...

Dec 30, 2020

Bruker announces software provider PMOD Technologies LLC

Nov 13, 2020

PMOD Technologies aims to equip researchers with best-in-class software tools for biomedical imaging research in humans...

Show all topics (10)

Hypoxia activates constitutive Luciferase reporter constructs

Jul 13, 2017

Studies in our laboratory sought to examine the hypoxic regulation of HIF activity in placental trophoblast cells, through the use of dual-reporter luciferase assays. Our study demonstrates that hypoxic conditions cause a significant increase in the level of constitutive luciferase reporter activity. We also show that this induction is not a cell-type or species-specific phenomenon and provides an alternative method for normalizing transfection efficiency in luciferase assays under hypoxic conditions. Our results suggest that in studies dealing with hypoxic conditions, caution should be used when interpreting measurements of transcriptional activity by traditional dual-reporter assays.

Read more

Scientific paper

Get more info