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Hypoxia activates constitutive Luciferase reporter constructs

Jul 13, 2017

Studies in our laboratory sought to examine the hypoxic regulation of HIF activity in placental trophoblast cells, through the use of dual-reporter luciferase assays. Our study demonstrates that hypoxic conditions cause a significant increase in the level of constitutive luciferase reporter activity. We also show that this induction is not a cell-type or species-specific phenomenon and provides an alternative method for normalizing transfection efficiency in luciferase assays under hypoxic conditions. Our results suggest that in studies dealing with hypoxic conditions, caution should be used when interpreting measurements of transcriptional activity by traditional dual-reporter assays.

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COY laboratory products

Coy Labs produces Glove Boxes, Anaerobic Chambers, and controlled atmosphere glove boxes.

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The flexible nature of the unit allows for the user to reach deeper and higher into the unit

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Gloveless anaerobic chambers offer the operator a convenient glove-free ability to handle and inspect samples under low oxygen (0-5 ppm) conditions

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