Accelerate to discover

Back to filter

Related topics

Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2

Cytek Biosciences

Nov 19, 2020

The development of a vaccine against COVID-19 is a hot topic for many research laboratories all over the world. Our aim...

Bruker announces software provider PMOD Technologies LLC

Bruker Biospin

Nov 13, 2020

PMOD Technologies aims to equip researchers with best-in-class software tools for biomedical imaging research in humans...

Microvascular injury in mild traumatic brain injury accelerates alzheimer-like pathogenesis in mice

RWD

Nov 9, 2020

Traumatic brain injury (TBI) is considered as the most robust environmental risk factor for Alzheimer’s disease (AD)....

Live animal imaging - Humane animal research

Bruker Biospin

Oct 26, 2020

Drawing on over 50 years of expertise in the life and animal sciences, Bruker designs preclinical imaging systems that...

Nov 23, 2020

Meet the Expert - Brent Barre interview

emka TECHNOLOGIES

Oct 21, 2020

Learn what a researcher at the forefront of COVID-19 research has to say about his current research environment.

Automated fever & hypothermia detection

emka TECHNOLOGIES

Oct 14, 2020

As one of the oldest clinical indicators of disease in mammals, temperature is a crucial parameter to monitor,...

Multi-modal molecular imaging driving pre-clinical research & pharmaceutical development

Bruker Biospin

Oct 6, 2020

Multi-modal molecular imaging at Massachusetts General Hospital – driving pre-clinical research and patient pathways...

Nov 23, 2020

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

Show all topics (10)

Imaging flow cytometry for automated detection of Hypoxia-Induced Erythrocyte shape change

Oct 31, 2016

We developed a new sickle imaging flow cytometry assay (SIFCA) and investigated its application. To perform the SIFCA, peripheral blood was diluted, deoxygenated (2% oxygen) for 2 hr, fixed, and analyzed using imaging flow cytometry. We developed a software algorithm that correctly classified investigator tagged "sickled" and "normal" erythrocyte morphology with a sensitivity of 100% and a specificity of 99.1%. The percentage of sickled cells as measured by SIFCA correlated strongly with the percentage of sickle cell anemia blood in experimentally admixed samples (R = 0.98, P ≤ 0.001), negatively with fetal hemoglobin (HbF) levels (R = -0.558, P = 0.027), negatively with pH (R = -0.688, P = 0.026), negatively with pretreatment with the antisickling agent, Aes-103 (5-hydroxymethyl-2-furfural) (R = -0.766, P = 0.002), and positively with the presence of long intracellular fibers as visualized by transmission electron microscopy (R = 0.799, P = 0.002). This study shows proof of principle that the automated, operator-independent SIFCA is associated with predictable physiologic and clinical parameters and is altered by the putative antisickling agent, Aes-103. SIFCA is a new method that may be useful in sickle cell drug development.

Read more

Scientific paper

Related technologies: Imaging flow cytometry

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Amnis

Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

Related products

Flow cytometer capable of high resolution microscopic assays

show detail

Combine the speed and statistical power of flow cytometry with microscopy

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey