Accelerate to discover

Back to filter

Related topics

Immunophenotyping Extracellular Vesicles using Amnis CellStream

Cytek Biosciences

May 29, 2020

Attempts to analyze EVs using traditional PMT- based flow cytometers has been hampered by the limit of detection of...

Four-week individual caging of male ICR mice alters body composition without change in body mass

MEDIKORS

May 25, 2020

Could individual caging affect the physical composition of outbred mice? To investigate this, dual X-ray absorptiometry...

Detect structural variants at 1% allele fraction: Dive deeper into heterogeneous cancer samples

BioNano Genomics

May 22, 2020

To get a complete picture of highly rearranged cancer genomes in heterogeneous samples, use Bionano genome imaging...

Insulin receptor substrate 1 is a substrate of the Pim protein kinases

Spectral Instruments Imaging

May 18, 2020

The goal of these studies was to identify Pim substrate(s) that could help define the pathway regulated by these...

May 29, 2020

Implementation of Micro CT in CAD/CAM dentistry for image processing and soft computing

Bruker Biospin

May 4, 2020

Present radiology devices are playing an important role in clinical dentistry but their area of application islimited...

Monitoring Lung tumor using Bioluminescence, Fluorescence and microCT

Bruker Biospin

Apr 14, 2020

Preclinical in vivo imaging have complementary relative strengths in sensitivity, resolution and molecular versus...

COVID‐19: Flow cytometry supporting the new challenge for global health

Cytek Biosciences

Apr 9, 2020

Several studies have described the biological and immunological features of the previous coronavirus epidemics, and...

May 29, 2020

Low-dose imaging in preclinical total-body PET/CT scanner

Bruker Biospin

Apr 8, 2020

Ionizing radiation constitutes a health risk to imaging scientists and study animals. Both PET and CT produce ionizing...

Show all topics (10)

Imaging flow cytometry for automated detection of Hypoxia-Induced Erythrocyte shape change

Oct 31, 2016

We developed a new sickle imaging flow cytometry assay (SIFCA) and investigated its application. To perform the SIFCA, peripheral blood was diluted, deoxygenated (2% oxygen) for 2 hr, fixed, and analyzed using imaging flow cytometry. We developed a software algorithm that correctly classified investigator tagged "sickled" and "normal" erythrocyte morphology with a sensitivity of 100% and a specificity of 99.1%. The percentage of sickled cells as measured by SIFCA correlated strongly with the percentage of sickle cell anemia blood in experimentally admixed samples (R = 0.98, P ≤ 0.001), negatively with fetal hemoglobin (HbF) levels (R = -0.558, P = 0.027), negatively with pH (R = -0.688, P = 0.026), negatively with pretreatment with the antisickling agent, Aes-103 (5-hydroxymethyl-2-furfural) (R = -0.766, P = 0.002), and positively with the presence of long intracellular fibers as visualized by transmission electron microscopy (R = 0.799, P = 0.002). This study shows proof of principle that the automated, operator-independent SIFCA is associated with predictable physiologic and clinical parameters and is altered by the putative antisickling agent, Aes-103. SIFCA is a new method that may be useful in sickle cell drug development.

Read more

Scientific paper

Related technologies: Imaging flow cytometry

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Amnis

Amnis has created the ImageStream Cell Analysis System, a breakthrough technology for high speed imaging and analysis of cells in flow.

Related products

Flow cytometer capable of high resoluition microscopic assays

show detail

Combine the speed and statistical power of flow cytometry with microscopy

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey