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Imaging human neural stem cells in culture

May 20, 2016

These are human neural stem cell cultured on an uncoated glass-bottom petri dish. Video courtesy of Lane Niles of Evan Snyder's lab at the Sanford Consortium for Regenerative Medicine.

The movie starts just after the cells are plated in the dish. Cells first aggregate into 'neurospheres', and then flatten on the surface, start to express exploratory lamellipodia and migrate around on the surface. Then they begin to proliferate and bulk up into multilayer colonies. Total time for each experiment is 6 days.

This is live biology. The illumination non-uniformity is caused by the strain defects in the plastic petri dish lid, always a problem with polystyrene.

These crisp, quality images were taken by the LumaScope inverted fluorescent microscope created by Etaluma, Inc.

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Related technologies: Time-lapse microscopy

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Etaluma

With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.

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The true color inverted brightfield microscopy with all of the LumaView software features of the fluorescent LumaScope

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The original single color 488nm excitation fluorescence inverted microscopy

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Lumascope 620 offers a larger sensor and 3-color fluorescence for the most powerful microscope offered anywhere at this price

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