Accelerate to discover

Back to filter

Related topics

STAT Protein Phosphorylation in CD4+ T Cells using the CellStream flow cytometer

Luminex

Jan 21, 2021

Cytokine-induced protein phosphorylation is a key cell signaling event within the immune system that modulates diverse...

Introducing Cytek cFluor, 14 Colors Immunoprofiling Kit

Cytek Biosciences

Jan 19, 2021

Cytek cFluor Immunoprofiling Kit allows for the identification of helper T cells, cytotoxic T cells, B cells, NK cells...

A Glimpse on physiological monitoring for the in vivo SKYSCAN Systems

Bruker Biospin

Jan 14, 2021

This past year has proven once again that in vivo imaging is an important aspect in the microCT world. For longitudinal...

Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting

Cytek Biosciences

Jan 11, 2021

In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...

Jan 21, 2021

CQ1 webinar : optimizing high-content imaging of 3D models for drug discovery

Yokogawa

Jan 7, 2021

In vitro spheroid models are fast becoming the de facto standard for drug discovery applications, largely due to their...

IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate

Sartorius

Jan 4, 2021

Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte platform for the in-vitro...

High gain mode now available on the Amnis ImageStreamX Mk II

Luminex

Dec 31, 2020

Increase camera gain during data acquisition and dramatically increase photonic sensitivity with High Gain mode, a new...

Jan 21, 2021

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 28, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Show all topics (10)

In vitro hypoxia studies using xCELLigence platform

Jun 14, 2016

Hypoxia, where the body or localized regions of the body are deprived of adequate oxygen supply, occurs during numerous acute and chronic disease states. Examples include reduced blood flow during heart attack or stroke, and the oxygen-restricted microenvironment within a tumor. Though cells can be studied in vitro under similar oxygen-poor conditions using a hypoxia chamber glove box, the inefficiencies of traditional endpoint assays are exacerbated by the constraints of working inside the chamber. In contrast, an xCELLigence instrument run within a hypoxia chamber will continuously provide a real-time assessment of cell number, cell size/morphology, cell attachment quality, and cell migration/invasion without the need for intermittent manipulation/intervention by the researcher.

Key Benefits of xCELLigence:
- Data quantity: Automatic real-time data acquisition makes it possible to collect, with ease, significantly more data points than what is possible using traditional end point assays. This is especially true for hypoxia studies because manual acquisition of end points inside a hypoxia chamber is difficult and laborious.
- Data quality and reproducibility are significantly better than what is possible using end point assays.
- Time savings: Once cells are plated, data can be acquired continuously for anywhere from minutes to days/weeks without the need for hands-on time by the researcher.

All seven xCELLigence instruments are compatible with in vitro hypoxia studies.

Application
Product news

Related technologies: Real-time, label free cell analysis

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

Located inside a tissue culture incubator, the RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey