Accelerate to discover

Back to filter

Related topics

Bruker webinar: Ear evolution in fossil mammals

Bruker Biospin

Nov 27, 2020

Hearing is just one of the functions aside of providing balance. Even juveniles obtain a fully developed ear region...

ONI Nanoimager joins the fight against COVID-19

ONI

Nov 24, 2020

ONI is supporting the global effort into research and diagnostics to tackle the COVID-19 pandemic. ONI is currently...

Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2

Cytek Biosciences

Nov 19, 2020

The development of a vaccine against COVID-19 is a hot topic for many research laboratories all over the world. Our aim...

Bruker announces software provider PMOD Technologies LLC

Bruker Biospin

Nov 13, 2020

PMOD Technologies aims to equip researchers with best-in-class software tools for biomedical imaging research in humans...

Nov 27, 2020

Microvascular injury in mild traumatic brain injury accelerates alzheimer-like pathogenesis in mice

RWD

Nov 9, 2020

Traumatic brain injury (TBI) is considered as the most robust environmental risk factor for Alzheimer’s disease (AD)....

Discover the new NovoCyte Penteon - when exceptional performance meets simplicity

Agilent technologies

Nov 6, 2020

The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte. You now have the flexibility to...

CS-FNX Hitachi Koki, discover the state-of-art ultracentrifuges series

Hitachi Koki

Nov 4, 2020

Hitachi Koki, as a world leading producer of centrifuges offers general purpose floor standing and table top...

Nov 27, 2020

Curiox webinar - how to retain more cells for Single Cell Sequencing and proteogenomics ?

Curiox

Oct 28, 2020

Do you think that centrifuging cells at 500 x g is not going to cause issues? Moreover, sample prep involving...

Show all topics (10)

Incucyte system used for generating single-cell-derived cancer-associated fibroblast clones

Jul 9, 2018

Tumours are composed of cancer cells and different types of stromal cells. Stromal cells help to create the special microenvironment which surrounds a tumour and therefore they affect proliferation, invasion and metastasis of tumours. From the stromal cells, CAFs are the most abundant. Even though they exhibit a homogenous morphology, phenotypically and functionally the CAFs are very heterogeneous. This makes in vivo and in Vitro studies more difficult with an indication that the cells are coming from various parts of the body. In the study, two primary CAF cultures from lung adenocarcinoma were transduced with human telomerase reverse transcriptase mutant. They were imaged on Incucyte and the number of successful single-cell origin colonies was counted. Then the expression of genes associated with tumour progression pathways was evaluated by qRT-PCR. As the results of the observation part, the researchers observed changes in lifespan and proliferation depending on the gene that was transduced in the cells. They also evaluated the mRNA levels of seven growth-factor related genes and three genes which were previously associated with pathways promoting tumour growth to study these phenomena further. This was done on fourteen clones and it was showed that the level of mRNA expression differs drastically between them. The study helps to create a deeper understanding of factor which functionally influences tumours and their progression. The method that the researchers developed can be further used to study clones and observe their effect on the cancerous microenvironment. 

 

See more

Scientific paper
Application

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey