Accelerate to discover

Back to filter

Related topics

Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

ACCELA educational webinar: Unlocking Spatial Biology with RareCyte Orion

RareCyte

Mar 16, 2023

Experience Spatial Biology with Orion Multiplex Imaging. Orion is a benchtop, high...

Recorded webinar: Anesthesia Considerations in Small Animal Imaging

Spectral Instruments Imaging

Mar 13, 2023

Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...

Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay

Cytek Biosciences

Mar 13, 2023

This kit has been designed to enumerate all major leukocyte subsets and it mirrors and expands on those identified in a...

Mar 24, 2023

Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II

RareCyte

Mar 7, 2023

CyteFinder II Instruments are high speed, whole slide imaging systems with options for liquid biopsy analysis and...

NanoCellect Webinar: Optimizing the Cell Line Development Process with Microfluidic Cell Sorting

NanoCellect

Mar 3, 2023

Single-cell selection and cloning are required for bioengineering workflows such as antibody production, cell therapy,...

New Kit for Bacterial analysis expands capability of Guava Muse flow cytometer

Luminex

Feb 20, 2023

With the Guava Muse cell analyzer, you can now achieve highly quantitative results at a fraction of the price, effort,...

Mar 24, 2023

Flash news - did you know, that you can use Singulator S100 also for plant research?

S2 Genomics

Feb 10, 2023

In this short TechNote you can find how Singulator can help the data quality in Single cell sequencing - now even in...

Show all topics (10)

Incucyte system used for generating single-cell-derived cancer-associated fibroblast clones

Jul 9, 2018

Tumours are composed of cancer cells and different types of stromal cells. Stromal cells help to create the special microenvironment which surrounds a tumour and therefore they affect proliferation, invasion and metastasis of tumours. From the stromal cells, CAFs are the most abundant. Even though they exhibit a homogenous morphology, phenotypically and functionally the CAFs are very heterogeneous. This makes in vivo and in Vitro studies more difficult with an indication that the cells are coming from various parts of the body. In the study, two primary CAF cultures from lung adenocarcinoma were transduced with human telomerase reverse transcriptase mutant. They were imaged on Incucyte and the number of successful single-cell origin colonies was counted. Then the expression of genes associated with tumour progression pathways was evaluated by qRT-PCR. As the results of the observation part, the researchers observed changes in lifespan and proliferation depending on the gene that was transduced in the cells. They also evaluated the mRNA levels of seven growth-factor related genes and three genes which were previously associated with pathways promoting tumour growth to study these phenomena further. This was done on fourteen clones and it was showed that the level of mRNA expression differs drastically between them. The study helps to create a deeper understanding of factor which functionally influences tumours and their progression. The method that the researchers developed can be further used to study clones and observe their effect on the cancerous microenvironment. 

 

See more

Scientific paper
Application

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey