Accelerate to discover

Back to filter

Related topics

New technologies to uncover the molecular basis of disorders of sex development

BioNano Genomics

Aug 16, 2018

Newer approaches, such as whole-genome sequencing and whole-genome mapping have the potential to identify novel...

Functional Imaging

Bruker Biospin

Aug 9, 2018

We bring you the latest KOL interview on the Albira Si PET/SPECT/CT from Prof. John Prior, CHUV Lausanne. He describes...

Differentation displacement in acute myeloid leukemia through enhancer activation

Acea Biosciences

Aug 7, 2018

Blast cell differentation is inhibitited by pharmacological targeting of lysine specific demethylase 1 (LSD1). In this...

Great ape genomes laid bare

BioNano Genomics

Aug 3, 2018

Scientists have long been interested in the functional genetic differences that distinguish humans from other ape...

Aug 18, 2018

Dynamic PET imaging and special focus in cardiac kinetic analysis

Bruker Biospin

Aug 1, 2018

Micro-computed tomography, or micro-CT, is being employed by a rapidly increasing number of laboratories for bone...

Novel insights on the mechanical properties of IFs using LUMICKS’ technology

LUMICKS

Jul 30, 2018

The journal Science Advances has recently published the paper ‘Viscoelastic properties of vimentin originate from...

IncuCyte S3 Cell-by-Cell analysis software

Sartorius

Jul 27, 2018

The IncuCyte S3 Live-Cell Analysis System, "Cell-by-Cell Analysis" software module, and IncuCyte reagents provide a...

Aug 18, 2018

xCELLigence AccuWound 96 - Is your Wound Healing assay due for an upgrade?

Acea Biosciences

Jul 26, 2018

Cell migration and invasion are critical to many biological fields. Scratch assay with AccuWound 96 brings...

Show all topics (10)

Introducing IncuCyte antibody Internalization assays

Jan 22, 2018

Antigen-mediated antibody internalization plays an important role in several antibody-based therapeutics. There is a continued growing body of interest in the application of antibodies as therapeutic agents for delivery of treatments to sites or cells of interest with promise of increased efficacy and reduced side effect potential.The significance and impact of therapeutic antibodies as effective treatments for disease span across numerous areas such as autoimmunity, cancer and inflammation, cardiovascular and infectious diseases, and many other disorders.The evolving therapeutic impact of antibody internalization on the antibody discovery field and targeted-mediated internalization includes the delivery of highly toxic drugs to cancer cells via antibody drug conjugates (ADCs), removal or degradation of surface receptors from cancer cells (i.e. EGFR), and antibody immunotherapies used to identify tumor cells for immune cell killing (i.e. ADCC or ADCP). Additionally, measuring and optimizing functional responses to antibodies, such as antibody clearance is of importance. Pinocytosis, which is one of the main elimination routes of antibodies, requires antibody optimization for qualitative pharmacokinetic measurements during antibody development. As each approach requires a series of antibody features, for example, to enable maintenance on the cell surface for identification of tumor cells, or for rapid internalization when delivering ADC’s, it is important to understand the uptake profile and clearance of antibody candidates for optimal antibody engineering and internalization characteristics.

Current approaches are limited by requiring labor-intensive labeling of each antibody, washing steps, and/or single-time point analysis. This can lead to unnecessary and lengthy processes, loss of cells, or missed and incorrect measurements due to limitations of single time-point analysis. Therefore, throughout the entire antibody screening and functional characterization process, there is a strong need to have an efficient, affordable method to fully and rapidly quantify antibody internalization using real time live-cell analysis.Essen Bioscience is proud to offer a new, novel solution that is compatible with both human and mouse test antibodies (utilizing IncuCyte Human and Mouse IgG1 FabFluor red antibody labeling reagent) for rapid, kinetic, high-throughput analysis of antibody internalization.

Read more

Scientific paper
Application
Product news
Video

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

Live Cell Imaging in Your Incubator

show detail

Real-time automated measurements of cell health, movement and function, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey