Accelerate to discover

Back to filter

Related topics

Cytek webinar : Evaluating Spectral Cytometry for Immune Profiling in Viral Disease

Cytek Biosciences

Apr 19, 2021

In conventional fluorescence cytometry, each fluorophore in a panel is measured in a target detector, through the use...

Rethinking 3D cell culture with IncuCyte: advanced cell cultures from iPSCs and organoids

Sartorius

Apr 14, 2021

The world is neither flat nor two-dimensional, so why should cell culture be? Traditional cell culture systems grow...

The impact of environmental Oxygen content on Natural Killer Cell Immunotherapy

Xcell Biosciences

Apr 13, 2021

Natural Killer (NK) cells are being studied in the clinic in a number of settings, including solid tumor...

Observe the movement of live cells that make up living tissue in vivo

IVIM Technology

Apr 8, 2021

IntraVital Microscopy is a technique that enables you to directly observe the movement of live cells that make up...

Apr 21, 2021

Cytek webinar recording : Improved flow cytometry assay resolution with autofluorescence extraction

Cytek Biosciences

Mar 30, 2021

The power of autofluorescence extraction in the detection of dim fluorescence on highly autofluorescent cells, and the...

Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

Sage Science

Mar 24, 2021

Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

Apr 21, 2021

Bruker webinar : High-resolution exploration of ESM-based vascularized tissue-flaps

Bruker Biospin

Mar 22, 2021

By employing a tissue engineering approach based on biological tissue constructs, Dr. Redenski has fabricated composite...

Show all topics (10)

Introducing IncuCyte antibody Internalization assays

Jan 22, 2018

Antigen-mediated antibody internalization plays an important role in several antibody-based therapeutics. There is a continued growing body of interest in the application of antibodies as therapeutic agents for delivery of treatments to sites or cells of interest with promise of increased efficacy and reduced side effect potential.The significance and impact of therapeutic antibodies as effective treatments for disease span across numerous areas such as autoimmunity, cancer and inflammation, cardiovascular and infectious diseases, and many other disorders.The evolving therapeutic impact of antibody internalization on the antibody discovery field and targeted-mediated internalization includes the delivery of highly toxic drugs to cancer cells via antibody drug conjugates (ADCs), removal or degradation of surface receptors from cancer cells (i.e. EGFR), and antibody immunotherapies used to identify tumor cells for immune cell killing (i.e. ADCC or ADCP). Additionally, measuring and optimizing functional responses to antibodies, such as antibody clearance is of importance. Pinocytosis, which is one of the main elimination routes of antibodies, requires antibody optimization for qualitative pharmacokinetic measurements during antibody development. As each approach requires a series of antibody features, for example, to enable maintenance on the cell surface for identification of tumor cells, or for rapid internalization when delivering ADC’s, it is important to understand the uptake profile and clearance of antibody candidates for optimal antibody engineering and internalization characteristics.

Current approaches are limited by requiring labor-intensive labeling of each antibody, washing steps, and/or single-time point analysis. This can lead to unnecessary and lengthy processes, loss of cells, or missed and incorrect measurements due to limitations of single time-point analysis. Therefore, throughout the entire antibody screening and functional characterization process, there is a strong need to have an efficient, affordable method to fully and rapidly quantify antibody internalization using real time live-cell analysis.Essen Bioscience is proud to offer a new, novel solution that is compatible with both human and mouse test antibodies (utilizing IncuCyte Human and Mouse IgG1 FabFluor red antibody labeling reagent) for rapid, kinetic, high-throughput analysis of antibody internalization.

Read more

Scientific paper
Application
Product news
Video

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey