Accelerate to discover

Back to filter

Related topics

Bionano Genomics news presented at AGBT 2019

BioNano Genomics

Apr 23, 2019

2019 is starting off with a bang for Bionano with higher throughput on Saphyr, easier and faster sample prep, detection...

Synthesis and characterization of functional nanofilm-coated live Immune cells using CQ1

Yokogawa

Apr 10, 2019

Layer-by-layer assembly techniques have been extensively studied in cell biology because of their simplicity of...

Product update: The high pass plus cassette

Sage Science

Apr 9, 2019

The high pass plus gel cassette is the newest addition to the Pippin family. It is dedicated to the BluePippin...

Novel imaging-based assay for hERG channel inhibition using iPS-derived Cardiomyocytes

Yokogawa

Apr 4, 2019

A large percentage of drugs fail in clinical studies due to cardiac toxicity, such as drug-induced QT...

Apr 24, 2019

Modified Beta-carotene-bound albumin nanoparticles for breast tumor ablation

Spectral Instruments Imaging

Mar 29, 2019

The albumin nanoparticles were fabricated using a beta-carotene cross-linker via the Nab technique. These new albumin...

Spectral flow cytometric analysis of loss-of-function mutations in granular cell tumors

Cytek Biosciences

Mar 29, 2019

Granular cell tumors (GCTs) are rare tumors that can arise in multiple anatomical locations,and are characterized by...

IncuCyte white paper: live-cell analysis for Neuroscientists

Sartorius

Mar 20, 2019

Stem cell technologies using iPSCs allow researchers to create differentiated neurons and support cells to study human...

Apr 24, 2019

Cell plasticity associated to circulating tumor cells from prostate cancer patients

Fluxion Biosciences

Mar 13, 2019

Treatment resistance is associated with tumor cells molecular plasticity. In metastatic castration resistant prostate...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids

Aug 3, 2017

Tumor spheroids exhibit more relevant morphology and increased cell survival compared to 2D cultures and have a hypoxic core. Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.
In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Product news

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

Real-time automated measurements of cell health, movement and function, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey