Accelerate to discover

Back to filter

Related topics

Introducing Cytek Cloud

Cytek Biosciences

Dec 1, 2022

Cytek Cloud is the new digital ecosystem that supports full spectrum flow cytometry research from panel design to data...

See More, Sort More with Cytek Full Spectrum Systems

Cytek Biosciences

Nov 28, 2022

Still wondering whether to join the shift to full spectrum cytometry?  Easily transfer assays from your Cytek Northern...

Demonstrate the value of RareCyte Orion system via web-based Minerva viewer

RareCyte

Nov 25, 2022

The HTA CRC Atlas X dataset contains images and other data being used for construction of an atlas of human colorectal...

All inside your incubator: xCELLigence eSight

Agilent technologies

Nov 3, 2022

Multiplex Live cell imaging and real-time biosensor measurement with xCELLigence eSight

Dec 8, 2022

RareCyte Orion system advances Multi-modal digital pathology for colorectal cancer diagnosis

RareCyte

Oct 19, 2022

Precision medicine is critically dependent on better methods for diagnosing and staging disease and predicting drug...

Introducing MARS system : revolutionary solution for your workflow of cell separation and enrichment

Applied Cells

Oct 7, 2022

The MARS family of instruments provides a breakthrough solution to complete the workflow of cell separation and...

AMI HTX installation in CEMEX Iasi, Romania

Spectral Instruments Imaging

Sep 30, 2022

Presentation movie

Dec 8, 2022

Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging

RareCyte

Sep 13, 2022

Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids

Aug 3, 2017

Tumor spheroids exhibit more relevant morphology and increased cell survival compared to 2D cultures and have a hypoxic core. Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.
In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Product news

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey