Accelerate to discover

Back to filter

Related topics

Introducing Celloger Mini: Automated and compact Live cell imaging inside your incubator

Curiosis

Jul 23, 2021

Celloger Mini is an automated live cell imaging system based on bright-field microscopy with fully motorized stages. 

High quality Bioluminescence and X-Ray analysis in lung squamous cell carcinoma

Spectral Instruments Imaging

Jul 21, 2021

Spectral Instruments Imaging provides unrivaled sensitivity for bioluminescence, fluorescence and X-Ray for in vivo...

Webinar recording: Autofluorescence management - the power of Spectral Flow Cytometry

Cytek Biosciences

Jul 15, 2021

One of the key advantages of spectral flow cytometry is its ability to extract sample autofluorescence, making it much...

Top 10 Pharma companies have chosen CYTEK for their flow cytometry facility

Cytek Biosciences

Jul 13, 2021

6 of Top 10 Pharma Companies (Based on total group revenues) have chosen CYTEK - full spectrum flow cytometer for their...

Jul 31, 2021

Introducing IZON Exoid: accurate analysis of nanoparticles from 1 to 1000 nm

IZON

Jul 7, 2021

The IZON Exoid is the only standardisable method of measuring the entire nano range (1–1000 nm).

Webinar recording: Super-Resolution imaging of Chromatin organization in health and disease

ONI

Jul 6, 2021

Understanding how cells organize the array of components within their membranous confines can not only provide insight...

Jul 31, 2021

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jun 23, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma. BEZ235 is an effective dual...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Feb 12, 2018

Current methods for assessing the growth and shrinkage  of  tumor  spheroids  are  limited  by  one  or  more  of  the  following:  (1)  assay  workflows  that  are  time-consuming,  expensive  or  laborious,  (2)  a  requirement  to  label  the  cells  (e.g.  a  fluorescent  probe)  which  may  perturb  the  biology  and  may  not  be  amenable  to  primary  tissue,  (3)  single  time-point  readouts  that  do  not  report  the  full  timecourse,  (4)  indirect  readouts (e.g. ATP) that may overlook  valuable morphological insight and/or mis-report cell growth.In  this  application  note  we  describe  methods  and  validation  data  for  miniaturized  (96/384-well)  live-cell  tumor  spheroid  assays   that   are   based   on   non-invasive   bright-field   image   analysis  performed  with  the  IncuCyte  S3  Spheroid  software  module.  Tumor  spheroids  are  formed  in  ultra-low  attachment  (ULA) plates and monitored for size and morphology for up to 2  weeks.  These  assays  are  flexible,  simple  to  run  and  provide  automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Application
Product news

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius & Essen BioScience

Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey