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Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Feb 12, 2018

Current methods for assessing the growth and shrinkage  of  tumor  spheroids  are  limited  by  one  or  more  of  the  following:  (1)  assay  workflows  that  are  time-consuming,  expensive  or  laborious,  (2)  a  requirement  to  label  the  cells  (e.g.  a  fluorescent  probe)  which  may  perturb  the  biology  and  may  not  be  amenable  to  primary  tissue,  (3)  single  time-point  readouts  that  do  not  report  the  full  timecourse,  (4)  indirect  readouts (e.g. ATP) that may overlook  valuable morphological insight and/or mis-report cell growth.In  this  application  note  we  describe  methods  and  validation  data  for  miniaturized  (96/384-well)  live-cell  tumor  spheroid  assays   that   are   based   on   non-invasive   bright-field   image   analysis  performed  with  the  IncuCyte  S3  Spheroid  software  module.  Tumor  spheroids  are  formed  in  ultra-low  attachment  (ULA) plates and monitored for size and morphology for up to 2  weeks.  These  assays  are  flexible,  simple  to  run  and  provide  automated and direct measures of tumor size in real-time.

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Essen BioScience & Sartorius is a team of engineers and biologists with deep expertise in cell-based assays and biomolecular interaction analysis. They invent, manufacture, supply and support instrumentation, reagents and protocols.

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