Optical tweezers, Confocal microscopy, STED nanoscopy, Acoustic force spectroscopy for single-molecule biophysics.
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Method to quantify local force distribution within biomolecular systems
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By using optical tweezers coupled with fluorescence microscopy, Dr. King et al. (2018) studied the ability of cyanine intercalator dyes to quantify the molecular tension within biomolecular structures. They found that intercalators can be used in biomolecular systems in real-time to measure the tension locally and to determine how applied forces are partitioned. As an example, the authors demonstrated that stretched and entwined DNA structures can generate transient DNA-DNA interactions that mimic DNA bridges, by measuring the tension and local force distribution within the structures.
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