Accelerate to discover

Back to filter

Related topics

IncuCyte webinar recording: Human Neurons in vitro, next-gen models for neurological disease

Sartorius

Apr 23, 2019

The ability to generate and manipulate human neurons in vitro is an exciting advance that enables the validation,...

Bionano Genomics news presented at AGBT 2019

BioNano Genomics

Apr 23, 2019

2019 is starting off with a bang for Bionano with higher throughput on Saphyr, easier and faster sample prep, detection...

Synthesis and characterization of functional nanofilm-coated live Immune cells using CQ1

Yokogawa

Apr 10, 2019

Layer-by-layer assembly techniques have been extensively studied in cell biology because of their simplicity of...

Product update: The high pass plus cassette

Sage Science

Apr 9, 2019

The high pass plus gel cassette is the newest addition to the Pippin family. It is dedicated to the BluePippin...

Apr 24, 2019

Comprehending diseased cardiac mechanics using speckle tracking echocardiography

VisualSonics FujiFilm

Apr 4, 2019

Are you working with high ultrasound imaging and are you interesting in speckle tracking echocardiography (STE)? Watch...

Novel imaging-based assay for hERG channel inhibition using iPS-derived Cardiomyocytes

Yokogawa

Apr 4, 2019

A large percentage of drugs fail in clinical studies due to cardiac toxicity, such as drug-induced QT...

Modified Beta-carotene-bound albumin nanoparticles for breast tumor ablation

Spectral Instruments Imaging

Mar 29, 2019

The albumin nanoparticles were fabricated using a beta-carotene cross-linker via the Nab technique. These new albumin...

Apr 24, 2019

Spectral flow cytometric analysis of loss-of-function mutations in granular cell tumors

Cytek Biosciences

Mar 29, 2019

Granular cell tumors (GCTs) are rare tumors that can arise in multiple anatomical locations,and are characterized by...

Show all topics (10)

Method to simultaneously construct 12 differently sized Illumina Nextera long mate pair libraries

Mar 15, 2016

The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA.

Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.

Scientists from The Genome Analysis Centre report a protocol using the SageELF to improve library preparation for long mate-pair sequencing. Using a Nextera kit, they show that generating 12 fractions with SageELF saves money and time while requiring less DNA input compared to other gel-based sizing options. Find more information in Heavens et al. (2015): A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost. In Bio Techniques: 59: 42-45.

Read the application note.

Scientific paper
Application
Product news

Related technologies: Targeted DNA/protein size collection

Brand profile

Sage Science

Preparative electrophoresis systems for targeted size selection of biomolecules.

Related products

Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

show detail

Targeted Size Selection for Next-Gen Sequencing (90bp – 50kb)

show detail

Automated size selection for 24 samples at a time.

show detail

Whole Sample Fractionation of DNA or Protein - Collect 12 Contiguous Fractions from a Single Sample

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey