Accelerate to discover

Back to filter

Related topics

The fastest qPCR thermal cycler in the world

BJS Biotechnologies

Sep 19, 2019

With a thermal ramp rate of up to 10°C per second, the xxpress NGx is the fastest, real-time thermal cycler in the...

Cytek webinar - the new age of spectral flow cytometry

Cytek Biosciences

Sep 9, 2019

The Cytek Aurora flow cytometer has made more than twenty-four color flow cytometry panels possible with only three...

New study resolves helicase dynamics and interactions during DNA replication using C-Trap

LUMICKS

Aug 30, 2019

Researchers from Rockefeller University used a C-Trap optical tweezers – fluorescence microscopy system to observe the...

Low-dose maging in a new preclinical total-body PET/CT scanner

Bruker Biospin

Aug 28, 2019

Ionizing radiation constitutes a health risk to imaging scientists and study animals. Both PET and CT produce ionizing...

Sep 22, 2019

Novel methods to analyze platelet activation with the NovoCyte flow cytometer

Acea Biosciences

Aug 26, 2019

Platelets are of large importance in medicine both for their essential role in hemostasis and thrombosis, as well as...

Bruker Plans to Develop World’s Highest Field Preclinical MRI System for Oncology and Neuroscience

Bruker Biospin

Aug 23, 2019

Bruker, a leading manufacturer of magnetic resonance imaging equipment, is working on the development of a powerful new...

Assessment of pro-arrhythmic effects using xCELLigence Cardio system and Pluricyte Cardiomyocytes

Acea Biosciences

Aug 21, 2019

The combination of Pluricyte Cardiomyocytes and the xCELLigence RTCA Cardio ECR system enables  detailed ...

Sep 22, 2019

Bruker announces acquisition of preclinical and molecular imaging research software provider PMOD

Bruker Biospin

Aug 19, 2019

The PMOD software is widely used for the analysis of positron emission tomography (PET) studies in neurology,...

Show all topics (10)

Method to simultaneously construct 12 differently sized Illumina Nextera long mate pair libraries

Mar 15, 2016

The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA.

Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.

Scientists from The Genome Analysis Centre report a protocol using the SageELF to improve library preparation for long mate-pair sequencing. Using a Nextera kit, they show that generating 12 fractions with SageELF saves money and time while requiring less DNA input compared to other gel-based sizing options. Find more information in Heavens et al. (2015): A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost. In Bio Techniques: 59: 42-45.

Read the application note.

Scientific paper
Application
Product news

Related technologies: Targeted DNA/protein size collection

Brand profile

Sage Science

Preparative electrophoresis systems for targeted size selection of biomolecules.

Related products

Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

show detail

Targeted Size Selection for Next-Gen Sequencing (90bp – 50kb)

show detail

Automated size selection for 24 samples at a time.

show detail

Whole Sample Fractionation of DNA or Protein - Collect 12 Contiguous Fractions from a Single Sample

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey